beta Galactosidase Antibody

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Product Details

Summary
Reactivity EcSpecies Glossary
Applications WB, DB
Clonality
Polyclonal
Host
Chicken
Conjugate
Unconjugated

Order Details

beta Galactosidase Antibody Summary

Description
Purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against stated buffer.
Immunogen
Prepared from eggs of chickens laid after repeated immunizations with full length native beta Galactosidase isolated from E.coli
Specificity
Cross reactivity against Beta Galactosidase from other tissues and species may occur but have not been specifically determined. Very low background staining has been reported in various assays.
Isotype
IgY
Clonality
Polyclonal
Host
Chicken
Purity
Multi-step
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Applications/Dilutions

Dilutions
  • Dot Blot
  • Western Blot
Application Notes
This antibody has been tested for use in ELISA and immunoblotting. Although not tested, this antibody may also be useful for neutralization assays, immunohistochemistry, flow Cytometry, immunoprecipitation, immunodiffusion, conjugation and most immunological methods requiring high titer and specificity.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Multi-step

Alternate Names for beta Galactosidase Antibody

  • EC 3.2.1.23; b-gal

Background

Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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