beta Galactosidase Antibody (2E9)

Product Discontinued

beta Galactosidase Antibody (2E9) Summary

• Immunogen: Full length protein (E. coli).
• Specificity: beta Galactosidase (2E9)
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunoassay
• Application Notes: Immunoassay Detection of b-galactosidase expressed by LacZ gene in cloned sequences.

Reactivity Notes

Reacts with b-galactosidase of E.coli and Aspergillus oryzae. Cross-reactivity with b-galactosidase from bovine liver is less than 10%.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Purity: Protein A purified

Alternate Names for beta Galactosidase Antibody (2E9)

• EC 3.2.1.23; b-gal

Background

Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for beta Galactosidase Antibody (NB110-7958)(1)

Publication using NB110-7958

• Chung S, Kim S, Andrew DJ. Uncoupling apical constriction from tissue invagination. Elife 2017-03-06 [PMID: 28263180]