Bartonella henselae Antibody (H2A10)

Product Discontinued

Bartonella henselae Antibody (H2A10) Summary

• Immunogen: Bartonella henselae
• Specificity: This identifies Bartonella henselae. Cross-reactivity tests were performed on B. henselae, B. quintana, B. bacilliformis, B. elizabethae, B. grahamii, B. taylorii, B. doshiae, and B. vinsonii strains. Reactivity was only obtained with Bartonella henselae.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
• Application Notes: IHC: For an ABC system, incubate for 30-60 minutes at RT. For formalin fixed paraffin embedded sections, perform heat mediated antigen retrieval before commencing with IHC. Bartonella henselae is a very small rod-shaped bacteria and is much easier to identify these under the microscope by using a red chromogen such as AEC. A magnification of 100 X (oil) is recommended for positive identification. Immunocytochemistry/Immunofluorescence was reported in scientific literature.

Reactivity Notes

Human and Bartonella henselae. Does not react with Helicobacter pylori and M. tuberculosis.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: Sodium Azide
• Purity: Tissue culture supernatant

Alternate Names for Bartonella henselae Antibody (H2A10)

• Bartonella henselae
• Bartonella spp.
• Cat Scratch fever
• Cat Scratch

Background

The causative bacterial agent of cat scratch disease has been identified as Bartonella henselae. In the past, complicated silver stains and/or PCR were used to identify and confirm this agent. This monoclonal antibody identifies Bartonella henselae in formalin-fixed paraffin-embedded tissues. Cross-reactivity tests were performed on (12) Bartonella henselae strains, (11) Bartonella quintana strains, (2)Bartonella bacilliformis strains, (1) B. elizabethae, (1) B. grahamii, (1) B. taylorii, (1) B. doshiae, and (1) B. vinsonii strains. Reactivity was only obtained with Bartonella henselae.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Bartonella henselae Antibody (NB600-1208)(1)

Publication using NB600-1208

• Sommese L, Pagliuca C, Avallone B et al. Evidence of Bacteroides fragilis Protection from Bartonella henselae-Induced Damage PLoS One 2012-01-01 [PMID: 23166739] (ICC/IF, Human)