Barmotin/7H6 antigen Antibody (7H6)

Product Discontinued

Barmotin/7H6 antigen Antibody (7H6) Summary

• Immunogen: A bile canaliculus-rich fraction was prepared from the livers of 8-wk-old Fischer 344 (F344) rats.
• Marker: Intercellular Junctions/Tight Junction Marker
• Specificity: The monoclonal antibody 7H6 reacts with barmotin. This protein is preferentially localized at the periphery of tight junctions of hepatocytes and other epithelia. The 7H6 antigen is also detectable in the intestine and kidney.
• Isotype: IgM
• Clonality: Monoclonal
• Host: Mouse
• Purity: IgM purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: The antibody can be used for Western blotting and immunohistology on frozen sections.For Western blotting and immunohistology dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 0.2 um filtered sterile antibody solution in PBS and 0.2% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.1 mg/ml
• Purity: IgM purified

Background

Tight junctions function to maintain cellular polarity and permeability barriers in epithelial and endothelial cells. Several molecules of the tight junction complex have been identified. The monoclonal antibody 7H6 reacts with a 155 kD tight junction-associated protein also designated barmotin. This protein is preferentially localized at the periphery of tight junctions of hepatocytes and other epithelia. The 7H6 antigen is also detectable in the intestine and kidney. Studies with Madin-Darby canine kidney (MDCK) cells indicate the 7H6 antigen to be an important molecule for regulation of the barrier function of tight junctions. Confocal laser scanning microscopy revealed that the expression of 7H6 antigen decreased sequentially during hepatocarcinogenesis. The 7H6 antigen was expressed intensely at the apical and basolateral membrane of cancer cells with glandular pattern. Examination of human gastric and colon cancer tissues showed that tight junctions were maintained highly in the well-differentiated (gland-forming) adenocarcinomas, but reduced in poorly-differentiated adenocarcinomas. In MDCK cells the disappearance of 7H6 expression is closely related with cell spreading in vitro induced hepatocyte growth factor/scatter factor. This antibody reacts with the human, canine, mouse, rat and chicken protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.