Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK(Dnp)-OH (Catalog # ES005). The specific activity is >12,000 pmol/min/µg, as measured with 10 µM ES005 and 2 ng enzyme in 100 µL of 50 mM Tris, 10 mM CaCl2, 0.15 M NaCl, 0.05% Brij 35, pH 7.5.
Source
Geobacillus stearothermophilus-derived Thermolysin protein
Protein/Peptide Type
Natural Enzymes
Purity
>70%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.
Applications/Dilutions
SDS-PAGE
36 kDa, reducing conditions
Publications
Read Publications using 3097-ZN in the following applications:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 °C as supplied.
3 months, -20 to -70 °C under sterile conditions after opening.
Buffer
Supplied as a 0.2 μm filtered solution in Tris, NaCl, CaCl2 and Glycerol.
Purity
>70%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Bacterial Thermolysin Protein, CF
Thermolysin
Background
Thermolysin is a zinc protease that can be used to activate a number of proteases produced at R&D Systems (see product inserts for details). Its activity can be inhibited by phosphoramidon, 1,10-phenanthroline or EDTA.
Protease
Catalog #
[Thermolysin]
Inhibitor
rhADAM8
1031-AD
0.75 μg/mL
Phosphoramidon
rhEnterokinase
1585-SE
1.58 μg/mL
1, 10-Phenanthroline
rhFactor VII
2338-SE
6.25 μg/mL
1, 10-Phenanthroline
rhFactor XI
2460-SE
10 μg/mL
EDTA
rhHGF Activator
1514-SE
4.3 μg/mL
1, 10-Phenanthroline
rhKallikrein 1
2337-SE
1.0 μg/mL
1, 10-Phenanthroline
rhKallikrein 3/PSA
1344-SE
1.0 μg/mL
1, 10-Phenanthroline
rhKallikrein 4
1719-SE
1.0 μg/mL
1, 10-Phenanthroline
rhKallikrein 7
2624-SE
10 μg/mL
EDTA
rhKallikrein 11
1595-SE
10 μg/mL
EDTA
rhKallikrein 14
2626-SE
10 μg/mL
EDTA
rmMast Cell Protease-11
2857-SE
0.2 μg/mL
1, 10-Phenanthroline
rhPlasma Kallikrein
2497-SE
10 μg/mL
EDTA
rmPlasma Kallikrein
2498-SE
10 μg/mL
EDTA
hThrombin
2196-SE
42 μg/mL
1, 10-Phenanthroline
rmTryptase-5
2634-SE
0.2 μg/mL
1, 10-Phenanthroline
rmTryptase epsilon
2059-SE
0.3 μg/mL
1, 10-Phenanthroline
rhTryptase beta 2
3796-SE
0.1 μg/mL
1, 10-Phenanthroline
rmFactor VII
3178-PA
1.0 μg/mL
1, 10-Phenanthroline
rhProprotein Convertase 7
2984-SE
2.0 μg/mL
1, 10-Phenanthroline
rm Prss28
4808-SE
0.1 μg/mL
1, 10-Phenanthroline
rhFactor XIV/Protein C
3349-SE
2.3 μg/mL
1, 10-Phenanthroline
rmFactor XIV/Protein C
4885-SE
5.0 μg/mL
1, 10-Phenanthroline
Publications for Thermolysin (3097-ZN)(9)
We have publications tested in 2 confirmed species: Human, Mouse.
We have publications tested in 2 applications: Bioassay, Enzyme Assay.
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PRODUCT AVAILABILITY: Update Regarding the Evolving COVID-19 Situation
Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.
