Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK(Dnp)-OH (Catalog # ES005). The specific activity is >12,000 pmol/min/µg, as measured with 10 µM ES005 and 2 ng enzyme in 100 µL of 50 mM Tris, 10 mM CaCl2, 0.15 M NaCl, 0.05% Brij 35, pH 7.5.
Source
Geobacillus stearothermophilus-derived Thermolysin protein
Protein/Peptide Type
Natural Enzymes
Purity
>70%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.
Applications/Dilutions
Dilutions
Enzyme Activity
SDS-PAGE
36 kDa, reducing conditions
Publications
Read Publications using 3097-ZN in the following applications:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 °C as supplied.
3 months, -20 to -70 °C under sterile conditions after opening.
Buffer
Supplied as a 0.2 μm filtered solution in Tris, NaCl, CaCl2 and Glycerol.
Purity
>70%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Bacterial Thermolysin Protein, CF
Thermolysin
Background
Thermolysin is a zinc protease that can be used to activate a number of proteases produced at R&D Systems (see product inserts for details). Its activity can be inhibited by phosphoramidon, 1,10-phenanthroline or EDTA.
Protease
Catalog #
[Thermolysin]
Inhibitor
rhADAM8
1031-AD
0.75 μg/mL
Phosphoramidon
rhEnterokinase
1585-SE
1.58 μg/mL
1, 10-Phenanthroline
rhFactor VII
2338-SE
6.25 μg/mL
1, 10-Phenanthroline
rhFactor XI
2460-SE
10 μg/mL
EDTA
rhHGF Activator
1514-SE
4.3 μg/mL
1, 10-Phenanthroline
rhKallikrein 1
2337-SE
1.0 μg/mL
1, 10-Phenanthroline
rhKallikrein 3/PSA
1344-SE
1.0 μg/mL
1, 10-Phenanthroline
rhKallikrein 4
1719-SE
1.0 μg/mL
1, 10-Phenanthroline
rhKallikrein 7
2624-SE
10 μg/mL
EDTA
rhKallikrein 11
1595-SE
10 μg/mL
EDTA
rhKallikrein 14
2626-SE
10 μg/mL
EDTA
rmMast Cell Protease-11
2857-SE
0.2 μg/mL
1, 10-Phenanthroline
rhPlasma Kallikrein
2497-SE
10 μg/mL
EDTA
rmPlasma Kallikrein
2498-SE
10 μg/mL
EDTA
hThrombin
2196-SE
42 μg/mL
1, 10-Phenanthroline
rmTryptase-5
2634-SE
0.2 μg/mL
1, 10-Phenanthroline
rmTryptase epsilon
2059-SE
0.3 μg/mL
1, 10-Phenanthroline
rhTryptase beta 2
3796-SE
0.1 μg/mL
1, 10-Phenanthroline
rmFactor VII
3178-PA
1.0 μg/mL
1, 10-Phenanthroline
rhProprotein Convertase 7
2984-SE
2.0 μg/mL
1, 10-Phenanthroline
rm Prss28
4808-SE
0.1 μg/mL
1, 10-Phenanthroline
rhFactor XIV/Protein C
3349-SE
2.3 μg/mL
1, 10-Phenanthroline
rmFactor XIV/Protein C
4885-SE
5.0 μg/mL
1, 10-Phenanthroline
Publications for Thermolysin (3097-ZN)(9)
We have publications tested in 2 confirmed species: Human, Mouse.
We have publications tested in 2 applications: Bioassay, Enzyme Assay.
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