Bacterial Thermolysin Protein, CF

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Product Details

Summary
Reactivity BaSpecies Glossary
Applications Enzyme Activity
Format
Carrier-Free

Order Details

Bacterial Thermolysin Protein, CF Summary

Details of Functionality
Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK(Dnp)-OH (Catalog # ES005). The specific activity is >12,000 pmol/min/µg, as measured with 10 µM ES005 and 2 ng enzyme in 100 µL of 50 mM Tris, 10 mM CaCl2, 0.15 M NaCl, 0.05% Brij 35, pH 7.5.
Source
Geobacillus stearothermophilus-derived Thermolysin protein
Protein/Peptide Type
Natural Enzymes
Purity
>70%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

SDS-PAGE
36 kDa, reducing conditions
Publications
Read Publications using
3097-ZN in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
Buffer
Supplied as a 0.2 μm filtered solution in Tris, NaCl, CaCl2 and Glycerol.
Purity
>70%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Bacterial Thermolysin Protein, CF

  • Thermolysin

Background

Thermolysin is a zinc protease that can be used to activate a number of proteases produced at R&D Systems (see product inserts for details). Its activity can be inhibited by phosphoramidon, 1,10-phenanthroline or EDTA.

Protease Catalog # [Thermolysin] Inhibitor
rhADAM8 1031-AD 0.75 μg/mL Phosphoramidon
rhEnterokinase 1585-SE 1.58 μg/mL 1, 10-Phenanthroline
rhFactor VII 2338-SE 6.25 μg/mL 1, 10-Phenanthroline
rhFactor XI 2460-SE 10 μg/mL EDTA
rhHGF Activator 1514-SE 4.3 μg/mL 1, 10-Phenanthroline
rhKallikrein 1 2337-SE 1.0 μg/mL 1, 10-Phenanthroline
rhKallikrein 3/PSA 1344-SE 1.0 μg/mL 1, 10-Phenanthroline
rhKallikrein 4 1719-SE 1.0 μg/mL 1, 10-Phenanthroline
rhKallikrein 7 2624-SE 10 μg/mL EDTA
rhKallikrein 11 1595-SE 10 μg/mL EDTA
rhKallikrein 14 2626-SE 10 μg/mL EDTA
rmMast Cell Protease-11 2857-SE 0.2 μg/mL 1, 10-Phenanthroline
rhPlasma Kallikrein 2497-SE 10 μg/mL EDTA
rmPlasma Kallikrein 2498-SE 10 μg/mL EDTA
hThrombin 2196-SE 42 μg/mL 1, 10-Phenanthroline
rmTryptase-5 2634-SE 0.2 μg/mL 1, 10-Phenanthroline
rmTryptase epsilon 2059-SE 0.3 μg/mL 1, 10-Phenanthroline
rhTryptase beta 2 3796-SE 0.1 μg/mL 1, 10-Phenanthroline
rmFactor VII 3178-PA 1.0 μg/mL 1, 10-Phenanthroline
rhProprotein Convertase 7 2984-SE 2.0 μg/mL 1, 10-Phenanthroline
rm Prss28 4808-SE 0.1 μg/mL 1, 10-Phenanthroline
rhFactor XIV/Protein C 3349-SE 2.3 μg/mL 1, 10-Phenanthroline
rmFactor XIV/Protein C 4885-SE 5.0 μg/mL 1, 10-Phenanthroline

Publications for Thermolysin (3097-ZN)(9)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: Bioassay, EnzAct, Enzyme Assay, enzymatic assay.


Filter By Application
Bioassay
(2)
EnzAct
(2)
Enzyme Assay
(3)
enzymatic assay
(1)
All Applications
Filter By Species
Human
(4)
Mouse
(3)
All Species
Showing Publications 1 - 9 of 9.
Publications using 3097-ZN Applications Species
Y Hao, W Purtha, C Cortesio, H Rui, Y Gu, H Chen, EA Sickmier, P Manzanillo, X Huang Crystal structures of human procathepsin H PLoS ONE, 2018;13(7):e0200374. 2018 [PMID: 30044821] (enzymatic assay, Human) enzymatic assay Human
Hofherr A, Wagner C, Fedeles S, Somlo S, Kottgen M N-glycosylation determines the abundance of the transient receptor potential channel TRPP2. J Biol Chem, 2014;289(21):14854-67. 2014 [PMID: 24719335] (Enzyme Assay, Mouse) Enzyme Assay Mouse
Fortugno P, Bresciani A, Paolini C, Pazzagli C, El Hachem M, D'Alessio M, Zambruno G Proteolytic activation cascade of the Netherton syndrome-defective protein, LEKTI, in the epidermis: implications for skin homeostasis. J Invest Dermatol, 2011;131(11):2223-32. 2011 [PMID: 21697885] (EnzAct, Human) EnzAct Human
Kantyka T, Fischer J, Wu Z, Declercq W, Reiss K, Schroder J, Meyer-Hoffert U Inhibition of kallikrein-related peptidases by the serine protease inhibitor of Kazal-type 6. Peptides, 0;32(6):1187-92. 0 [PMID: 21439340] (Bioassay) Bioassay
Fernando S, Fletcher BS Targeting tumor endothelial marker 8 in the tumor vasculature of colorectal carcinomas in mice. Cancer Res., 2009;69(12):5126-32. 2009 [PMID: 19528090] (Enzyme Assay, Mouse) Enzyme Assay Mouse
Lilla JN, Joshi RV, Craik CS, Werb Z Active plasma kallikrein localizes to mast cells and regulates epithelial cell apoptosis, adipocyte differentiation, and stromal remodeling during mammary gland involution. J. Biol. Chem., 2009;284(20):13792-803. 2009 [PMID: 19297327] (Enzyme Assay, Mouse) Enzyme Assay Mouse
Ramani VC, Haun RS The extracellular matrix protein fibronectin is a substrate for kallikrein 7. Biochem. Biophys. Res. Commun., 2008;369(4):1169-73. 2008 [PMID: 18343220] (Bioassay, Human) Bioassay Human
Mukai S, Fukushima T, Naka D, Tanaka H, Osada Y, Kataoka H Activation of hepatocyte growth factor activator zymogen (pro-HGFA) by human kallikrein 1-related peptidases. FEBS J., 2008;275(5):1003-17. 2008 [PMID: 18221492] (EnzAct, Human) EnzAct Human
Gomez-Gaviro M, Dominguez-Luis M, Canchado J, Calafat J, Janssen H, Lara-Pezzi E, Fourie A, Tugores A, Valenzuela-Fernandez A, Mollinedo F, Sanchez-Madrid F, Diaz-Gonzalez F Expression and regulation of the metalloproteinase ADAM-8 during human neutrophil pathophysiological activation and its catalytic activity on L-selectin shedding. J. Immunol., 2007;178(12):8053-63. 2007 [PMID: 17548643]

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