Bacterial Counting Assay Kit (Colorimetric)

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Quantification : Bacterial Counting Assay Kit (Colorimetric) - Effect of time on E.coli growth: Density of bacteria as determined by time to yield change at OD 460 nm during 8 hr incubation at 37C.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP3-09183
    • Availability
      Product Discontinued

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Bacterial Counting Assay Kit (Colorimetric) Summary

Description
This Bacterial Counting Assay Kit (Colorimetric) is a simple and sensitive assay to quantify bacterial concentration and viability. The assay is based on the cleavage of a water soluble tetrazolium salt (WST) to formazan by mitochondrial dehydrogenases. The color produced is directly proportional to the number of viable bacteria and can be quantified using a microplate reader (OD 460 nm). The assay is sensitive and can detect the growth of low density cultures starting less than 10 bacteria per well at the time of adding the reagent. The assay can also be used to count the number of living bacteria in a broth culture or to study bacterial viability in response to inhibitors, types of media, antibiotics, growth and heterologous protein overexpression conditions.

This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab284554)
Specificity
Measure bacterial viability in response to changes in environment, growth activators, inhibitors, antibiotics, overexpression inducers etc. Screen bacteria to determine the toxicity of protein products.
Kit Type
Assay Kit (Colorimetric)

Applications/Dilutions

Dilutions
  • Quantification

Packaging, Storage & Formulations

Storage
Store at -20C.

Kit Components

Components
  1. Electrocoupling Solution (ECS) (5 mL)
  2. WST Reagent (lyophilized) (1 vial)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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