| Description | This Bacterial Counting Assay Kit (Colorimetric) is a simple and sensitive assay to quantify bacterial concentration and viability. The assay is based on the cleavage of a water soluble tetrazolium salt (WST) to formazan by mitochondrial dehydrogenases. The color produced is directly proportional to the number of viable bacteria and can be quantified using a microplate reader (OD 460 nm). The assay is sensitive and can detect the growth of low density cultures starting less than 10 bacteria per well at the time of adding the reagent. The assay can also be used to count the number of living bacteria in a broth culture or to study bacterial viability in response to inhibitors, types of media, antibiotics, growth and heterologous protein overexpression conditions.
This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab284554) |
| Specificity | Measure bacterial viability in response to changes in environment, growth activators, inhibitors, antibiotics, overexpression inducers etc. Screen bacteria to determine the toxicity of protein products. |
| Kit Type | Assay Kit (Colorimetric) |
| Storage | Store at -20C. |