Avidin D [Alkaline Phosphatase]

Avidin D [Alkaline Phosphatase] [A-2100-NB] - Alkaline Phosphatase Avidin D

• Applications: WB, ELISA, IHC, ISH, ESPOT, ICC/IF
• Conjugate: Alkaline Phosphatase

Avidin D [Alkaline Phosphatase] Summary

• Description: Features:
  
  
• Suitable for use in solid-phase assays, tissue/cell staining systems, and blotting applications.
• Specific covalent linkages are chosen to provide stable, highly active conjugates.
• One unit is 1 µmole p-nitrophenylphosphate hydrolyzed per min. at 37 °C, pH 9.8.

Applications/Dilutions

• Dilutions:
  • ELISA
  • ELISPOT
  • Immunocytochemistry
  • Immunohistochemistry
  • In-situ Hybridization
  • Western Blot
• Application Notes: To avoid adsorption or denaturing of the enzyme conjugate, dilutions may be made in 20 mM HEPES, 0.5 M NaCl, pH 7.5, containing 0.1 mg/mL IHC- grade BSA. DO NOT USE FRACTION V GRADE BSA. One unit equals 1.0 micromole of p-nitrophenol liberated from p-nitrophenylphosphate per minute at 37C (pH 9.5).
  
  Recommended concentration for use is 0.2 units/mL.
  
  Storage of dilute solutions of the enzyme conjugate is not recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Reconstitution Instructions: Reconstitute with 1 mL water. The resulting solution will have the following composition: 10 mM HEPES, 0.15 M NaCl, 0.08% sodium azide, 1 mM Mg++, 0.1 mM Zn++, pH 7.5. This product has been stabilized by addition of Ficoll and IHC-grade BSA.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.