Avidin D [Alkaline Phosphatase]

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Avidin D [Alkaline Phosphatase] [A-2100-NB] - Alkaline Phosphatase Avidin D

Product Details

Summary
Applications WB, ELISA, IHC, ISH, ESPOT, ICC/IF
Conjugate
Alkaline Phosphatase

Order Details

Avidin D [Alkaline Phosphatase] Summary

Description
Features:

  • Suitable for use in solid-phase assays, tissue/cell staining systems, and blotting applications.
  • Specific covalent linkages are chosen to provide stable, highly active conjugates.
  • One unit is 1 µmole p-nitrophenylphosphate hydrolyzed per min. at 37 °C, pH 9.8.

  • Applications/Dilutions

    Dilutions
    • ELISA
    • ELISPOT
    • Immunocytochemistry
    • Immunohistochemistry
    • In-situ Hybridization
    • Western Blot
    Application Notes
    To avoid adsorption or denaturing of the enzyme conjugate, dilutions may be made in 20 mM HEPES, 0.5 M NaCl, pH 7.5, containing 0.1 mg/mL IHC- grade BSA. DO NOT USE FRACTION V GRADE BSA. One unit equals 1.0 micromole of p-nitrophenol liberated from p-nitrophenylphosphate per minute at 37C (pH 9.5).

    Recommended concentration for use is 0.2 units/mL.

    Storage of dilute solutions of the enzyme conjugate is not recommended.

    Packaging, Storage & Formulations

    Storage
    Store at 4C. Do not freeze.
    Reconstitution Instructions
    Reconstitute with 1 mL water. The resulting solution will have the following composition: 10 mM HEPES, 0.15 M NaCl, 0.08% sodium azide, 1 mM Mg++, 0.1 mM Zn++, pH 7.5. This product has been stabilized by addition of Ficoll and IHC-grade BSA.

    Limitations

    This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

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    Product General Protocols

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    Video Protocols

    WB Video Protocol

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    Additional Avidin D Products

    Array A-2100-NB

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