Avidin Antibody [HRP]

Product Discontinued

Avidin Antibody [HRP] Summary

• Description: This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
• Immunogen: Avidin (Hen Egg White)
• Specificity: No cross reactivity occurs against Streptavidin.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Delipidation and Defibrination

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000-1:50000
  • Immunohistochemistry 1:500-1:2000
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:2000-1:5000
• Application Notes: NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).
• Readout System:
  • NovaLume Plus Chemiluminescent Reagent LUM-555

Reactivity Notes

No cross reactivity occurs against Streptavidin

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
• Preservative: 0.01% Gentamicin Sulfate
• Purity: Delipidation and Defibrination

Alternate Names for Avidin Antibody [HRP]

• AVD
• Avidin
• LOC396260

Background

The biological function of avidin is not known. It is a homotetrameric glycoprotein found in chicken egg white, and forms a strong non covalent specific complex with biotin (one molecule of biotin per subunit of avidin).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.