Aurora A/B Antibody

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Western Blot: Aurora A/B Antibody [NBP2-32777] - analysis of Aurora A/B in HCT-116 whole cell lysate using this antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.05 mg/ml

Order Details

Aurora A/B Antibody Summary

Immunogen
This antibody was developed against KLH-conjugated synthetic peptides corresponding to amino acids 2-16 and 157-170 of human ARK. These sequences are over 80% homologous in mouse and rat. These sequences are 100% homologous in ARK1 and ARK2.
Marker
Mitosis Marker
Specificity
In HCT-116, a 48 kDa band is observed.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot 2-4 ug/ml

Reactivity Notes

Reacts with Human. Predicted to react with Mouse and Rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.2% gelatin
Preservative
0.05% Sodium Azide
Concentration
0.05 mg/ml
Purity
Protein G purified

Background

The Aurora gene, a serine-threonine kinase, orginally discovered in Drosophila, has been found to be responsible for a chromosomal segregation defect. The mammalian homologues of Drosophila Aurora have been termed ARK1 (Aurora-related kinase 1) and ARK2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional Aurora A/B Products

Aurora A/B NBP2-32777

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