Aurora A/B Antibody

Western Blot: Aurora A/B Antibody [NBP2-32777] - analysis of Aurora A/B in HCT-116 whole cell lysate using this antibody.

Product Discontinued

Aurora A/B Antibody Summary

• Immunogen: This antibody was developed against KLH-conjugated synthetic peptides corresponding to amino acids 2-16 and 157-170 of human ARK. These sequences are over 80% homologous in mouse and rat. These sequences are 100% homologous in ARK1 and ARK2.
• Marker: Mitosis Marker
• Specificity: In HCT-116, a 48 kDa band is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 2-4 ug/ml

Reactivity Notes

Predicted to react with Mouse and Rat.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS containing 0.2% gelatin
• Preservative: 0.05% Sodium Azide
• Concentration: 0.05 mg/ml
• Purity: Protein G purified

Alternate Names for Aurora A/B Antibody

• AIK;AIK2;AIM1;AIM-1;AIRK1;AIRK2;ARK1;ARK2;AURA;AurB;AURKA;AURKB;aurkb-sv1;aurkb-sv2;AYK1;BTAK;IAK1;IPL1;PPP1R47;PPP1R48;STK1;STK12;STK15;STK5;STK6;STK7

Background

The Aurora gene, a serine-threonine kinase, orginally discovered in Drosophila, has been found to be responsible for a chromosomal segregation defect. The mammalian homologues of Drosophila Aurora have been termed ARK1 (Aurora-related kinase 1) and ARK2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.