ATPase Activity Activity Assay Kit (Colorimetric)

ATPase Activity Activity Assay Kit (Colorimetric) [NBP2-59733]

ATPase Activity Activity Assay Kit (Colorimetric) [NBP2-59733]

Product Discontinued

ATPase Activity Activity Assay Kit (Colorimetric) Summary

• Description: This kit provides a quick and easy method for monitoring ATPase activity in various samples. This ATPase assay hydrolyzes ATP releasing ADP and a free phosphate ion, and through linked reactions, a strong, stable chromophore is generated. The assay is designed to measure ATPase activity in biological samples with detection sensitivity ~5 mU/l. Detection method: Absorbance at 650 nm.
  
  This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab234055)
• Specificity: Rapid assessment of ATPase activity in biological samples or recombinant ATPase preparations.
• Kit Type: Activity Assay Kit (Colorimetric)

Applications/Dilutions

Publications

Read Publication using NBP2-59733.

Packaging, Storage & Formulations

• Storage: Store at -20C in the dark. Avoid freeze-thaw cycles.

Background

ATPase (Adenosine Triphosphatase: EC 3.6.1.3) is an important enzyme for maintaining the cell membrane potential, transporting ions and regulating cellular volume. It catalyzes the decomposition of ATP into ADP and a free phosphate ion. The hydrolysis of ATP is highly exergonic releasing energy that is utilized in several cellular processes. There are many classes of ATPases including Na+/K+ ATPase, H+/K+ ATPase and Ca2+ ATPase. The deficiency of mitochondrial ATPase is serious: for example, Na+/K+ ATPase deficiency increases anxiety-related behavior, while Ca2+ ATPase deficiency leads to exertional muscle pain syndrome. Therefore, accurate detection of ATPase activity is valuable for the diagnosis and mechanistic studies of some of these diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Publications for ATPase Activity Kit (NBP2-59733)(1)

Publication using NBP2-59733

• Yu J, Im H, Lee G Unwinding mechanism of SARS-CoV helicase (nsp13) in the presence of Ca2+, elucidated by biochemical and single-molecular studies Biochemical and biophysical research communications 2023-05-18 [PMID: 37235917]