ATPase Activity Activity Assay Kit (Colorimetric) Summary
This kit provides a quick and easy method for monitoring ATPase activity in various samples. This ATPase assay hydrolyzes ATP releasing ADP and a free phosphate ion, and through linked reactions, a strong, stable chromophore is generated. This assay can detect ATPase activity less than 0.005 U/L. Detection method: Absorbance at 650 nm.
Detects ATPase Activity less than 0.005 U/L
Activity Assay Kit (Colorimetric)
Packaging, Storage & Formulations
Store at -20C in the dark. Avoid freeze-thaw cycles.
- ATPase Assay Buffer
- ATPase Developer
- ATPase Positive Control
- ATPase Substrate
- Phosphate Standard (10 mM)
ATPase (Adenosine Triphosphatase: EC 18.104.22.168) is an important enzyme for maintaining the cell membrane potential, transporting ions and regulating cellular volume. It catalyzes the decomposition of ATP into ADP and a free phosphate ion. The hydrolysis of ATP is highly exergonic releasing energy that is utilized in several cellular processes. There are many classes of ATPases including Na+/K+ ATPase, H+/K+ ATPase and Ca2+ ATPase. The deficiency of mitochondrial ATPase is serious: for example, Na+/K+ ATPase deficiency increases anxiety-related behavior, while Ca2+ ATPase deficiency leads to exertional muscle pain syndrome. Therefore, accurate detection of ATPase activity is valuable for the diagnosis and mechanistic studies of some of these diseases.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed
for 6 months from date of receipt.
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