ATP Assay Kit (Colorimetric/Fluorometric)

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Product Details

Summary
Reactivity Hu, Mu, Rt, MaSpecies Glossary
Suitable Sample Type
Cell and tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids

Order Details

ATP Assay Kit (Colorimetric/Fluorometric) Summary

Description
Kit is designed to be a robust method which utilizes the phosphorylation of glycerol to generate a product that is easily quantified.
  • Detection method: Fluorescence plate reader (Ex/Em 535/587). Absorbance at 570 nm
  • Applications: The assay can detect as low as 50 picomol (1 UM) of ATP in various samples.
Kit Type
Assay Kit (Colorimetric/Fluorometric)

Applications/Dilutions

Publications
Read Publication using NBP2-54855.

Packaging, Storage & Formulations

Storage
Store at -20C.

Kit Components

Components
  1. Developer Mix (lyophilized)
  2. ATP Assay Buffer
  3. ATP Converter
  4. ATP Probe (in DMSO)
  5. ATP Standard (1 umol; lyophilized)

Background

ATP is the primary energy currency of living systems. Virtually all energy requiring processes utilize the chemical energy stored in the phosphate bond of ATP. ATP is formed exclusively in the mitochondria and a variety of genetic diseases can affect ATP formation. There are a number of commercially available ATP assays which detect femtomoles or less of ATP by measuring luminescence (Novus Kit 254-200, for example) but these kits require specialized luminescence instrumentation and utilize luciferase which can be difficult to maintain in active form. Novus' ATP Colorimetric and Fluorometric Assay kit is designed to be a robust, simple method which utilizes the phosphorylation of glycerol to generate a product that is easily quantified by colorimetric (WLmax = 570 nm) or fluorometric (Ex/Em = 535/587 nm) methods.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Publications for ATP Kit (NBP2-54855)(1)

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Other Available Formats

Additional ATP Products

Array NBP2-54855

Blogs on ATP.

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O-linked beta-N-acetylglucosamine (O-GlcNAc) is a sugar attachment to serine or threonine hydroxyl moieties on nuclear and cytoplasmic proteins. O-GlcNAc modified proteins are generally either cytoplasmic or nuclear proteins, and unlike asparagine-lin...  Read full blog post.

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