ATAD1 Antibody (S125-10)

Western Blot: ATAD1 Antibody (S125-10) [NBP2-12921] - analysis of Rat brain membrane lysate showing detection of ATAD1 ATPase protein using Mouse Anti-ATAD1 ATPase Monoclonal Antibody, Clone S125-10 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-ATAD1 ATPase Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Immunocytochemistry/Immunofluorescence: ATAD1 Antibody (S125-10) [NBP2-12921] - Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20C. Primary Antibody: Mouse Anti-ATAD1 ATPase Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Cytoplasmic and accumulates around nucleus.

Immunohistochemistry-Paraffin: ATAD1 Antibody (S125-10) [NBP2-12921] - Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-ATAD1 ATPase Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Nuclear staining in all basal cells.

Product Discontinued

ATAD1 Antibody (S125-10) Summary

• Immunogen: Fusion protein amino acids 1-361 (Full length) of mouse Thorase
• Localization: Peroxisome
• Specificity: Detects approx 40kDa.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ATAD1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.1-1.0 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 0.1-1.0 ug/ml
  • Microarray
  • Western Blot 1-10 ug/ml
• Application Notes: 1 ug/ml of Thorase/Atad1 Antibody was sufficient for detection of Thorase in 10 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for ATAD1 Antibody (S125-10)

• AFDC1
• ATPase family AAA domain-containing protein 1
• ATPase family, AAA domain containing 1
• FLJ14600

Background

1 ug/mL was sufficient for detection of Thorase in 10ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG: HRP as the secondary antibody.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ATAD1