Asparaginase Antibody [HRP]

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Western Blot: Asparaginase Antibody [HRP] [NB120-20848] - Lane 1: L-ASPARAGINASE. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase rabbit secondary antibody at 1:1,000 for 60 ...read more

Product Details

Summary
Reactivity BaSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Rabbit
Conjugate
HRP

Order Details

Asparaginase Antibody [HRP] Summary

Description
This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Immunogen
Asparaginase (E. coli).
Localization
Cytoplasmic and Secreted.
Specificity
Recognises purified and partially purified Asparaginase (E. coli).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ASPG
Purity
Ion exchange chromatography
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:5000
  • ELISA 1:10000-1:40000
Readout System

Reactivity Notes

Cross-reacts with E. coli.Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Gentamicin Sulfate
Purity
Ion exchange chromatography

Alternate Names for Asparaginase Antibody [HRP]

  • AnsA
  • AnsB
  • Colaspase
  • Cytoplasmic asparaginase I
  • L ASNase I
  • L ASNase II
  • L asparaginase 1
  • L asparaginase 2
  • L asparaginase I
  • L asparaginase II precursor
  • L asparaginase II
  • L asparagine amidohydrolase I
  • L asparagine amidohydrolase II

Background

Asparaginase is an enzyme purified from E. coli and Erwinia carotovora. It acts by deaminating extracellular L asparagine, an amino acid that appears to be essential for protein synthesis by some tumour cells which are unable to synthesise asparagine. Asparaginase from Erwinia carotovora is serologically and biochemically distinct from asparaginase from E. coli, although its antineoplastic activity and toxicity is similar. Asparaginase is usually considered to be cell cycle phase nonspecific, but it may block some cells in G1 or S phase. Asparaginase reduces cellular and humoral immunity. E.coli contains two L asparaginase isoenzymes: L asparaginase I, a low affinity enzyme located in the cytoplasm, and L asparaginase II, a high affinity secreted enzyme.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ASPG