Human ASCA Combi ELISA Kit (Colorimetric)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, NULL
Suitable Sample Type
Plasma, Serum
Sensitivity
0.5 U/mL

Order Details

Human ASCA Combi ELISA Kit (Colorimetric) Summary

Description
ASCA ELISA Kit is an indirect solid phase enzyme immunoassay for the quantitative measurement of IgG and IgA class autoantibodies against mannan from Saccharomyces cerevisiae in human serum or plasma.
Sensitivity
0.5 U/mL
Assay Type
Indirect ELISA
Inter-Assay
CV% < 6.30%
Intra-Assay
CV% < 6.13%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)

Applications/Dilutions

Dilutions
  • ELISA
  • Quantification
Publications
Read Publication using KA1270.

Packaging, Storage & Formulations

Storage
Store at 4C.

Kit Components

Components
  1. Calibrators A-F (0; 6.3; 12.5; 25; 50; 100 U/ml), containing serum/buffer matrix (PBS, BSA,detergent, NaN3 0.09%), yellow. Ready to use.
  2. Control positive (1) and negative (2), containing ASCA antibodies in a serum/buffer matrix(PBS, BSA, detergent, NaN3 0.09%), yellow, Ready to use. The concentration is specifiedon the certificate of analysis.
  3. Divisible microplate consisting of 12 modules of 8 wells each. Ready to use.
  4. Enzyme conjugate; containing anti-human IgA antibodies, HRP labelled; PBS, BSA,detergent, preservative ProClin 300 0.05%, light red. Ready to use.
  5. Enzyme conjugate; containing anti-human IgG antibodies, HRP labelled; PBS, BSA,detergent, preservative ProClin 300 0.05%, light red. Ready to use.
  6. Sample buffer P, containing PBS, BSA, detergent, preservative NaN3 0.09%, yellow,concentrate (5x).
  7. Stop solution; contains acid. Ready to use.
  8. TMB substrate; containing 3,3’,5,5’-Tetramethylbenzidin, colorless. Ready to use.
  9. Wash solution, containing Tris, detergent, preservative NaN3 0.09%; concentrate (50x).

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for ASCA Combi ELISA Kit (KA1270)(1)

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Product General Protocols

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FAQs for ASCA Combi ELISA Kit (KA1270). (Showing 1 - 1 of 1 FAQ).

  1. What is the difference between the Quantikine and Duoset ELISA kits?
    • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

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Array KA1270

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