Human Arachidonic Acid ELISA Kit (Colorimetric)

Human Arachidonic Acid ELISA Kit (Colorimetric) [NBP2-59872] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Human Arachidonic Acid ELISA Kit (Colorimetric) [NBP2-59872] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, cell culture supernates and tissue homogenates
• Standard Curve Range: 78.125 ng/mL - 5000 ng/mL
• Sensitivity: 69.748 ng/mL

Human Arachidonic Acid ELISA Kit (Colorimetric) Summary

• Description: Assay Length: 1-5 hours
• Specificity: This assay has high sensitivity and excellent specificity for detection of human AA. No significant cross-reactivity or interference between human AA and analogues was observed.
• Standard Curve Range: 78.125 ng/mL - 5000 ng/mL
• Sensitivity: 69.748 ng/mL
• Assay Type: Competitive ELISA
• Inter-Assay: %CV < 10
• Intra-Assay: %CV < 8
• Sample Volume: 50 - 100 uL
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Arachidonic Acid ELISA Kit (Colorimetric)

• 506-32-1 ELISA
• 506-32-1
• AA ELISA
• ARA
• Omega-6 Fatty Acid

Background

The minimum detectable dose of human AA is typically less than 69.748 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest human AA concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Arachidonic Acid ELISA Kit (NBP2-59872)(3)

Publications using NBP2-59872

• Xu C, Gu L, Hu L et al. FADS1-arachidonic acid axis enhances arachidonic acid metabolism by altering intestinal microecology in colorectal cancer Nature communications 2023-04-11 [PMID: 37041160]
• Hu N, Bai L, Dai E et al. Pirin is a nuclear redox-sensitive modulator of autophagy-dependent ferroptosis Biochemical and biophysical research communications 2020-12-26 [PMID: 33373853] (ELISA, Human)
• Baer Alan, Colon-Moran Winston, Bhattarai Nirjal. Characterization of the effects of immunomodulatory drug fingolimod (FTY720) on human T cell receptor signaling pathways. Sci Rep 2018-01-01 [PMID: 30026610] (ELISA, Human)

FAQs for Arachidonic Acid ELISA Kit (NBP2-59872). (Showing 1 - 1 of 1 FAQs).

1. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.