Apoptotic Cell Isolation Kit Summary
Rapid and efficient kit for isolating apoptotic cells or removing dead cells from cell culture or tissue preparations using Annexin V magnetic beads.
- Detection method: Magnetic bead bound apoptotic cells can be separated from normal cells.
- Applications: Kit provides a simple and efficient means for isolation of apoptotic cells from normal culture using annexin V magnetic beads. The separated apoptotic cells and normal cells can be used in a variety of assays to study apoptotic mechanisms and pathways. The kit has also been successfully used to remove dead cells from normal culture.
The magnetic separating rack optimized for this kit is catalog number NBP2-59064.
Packaging, Storage & Formulations
- Apoptotic Cell Releasing Buffer
- 1X Binding Buffer
- Annexin V-Biotin
- Streptavidin-magnetic beads
The Apoptotic Cell Isolation Kit provides a simple and efficient means for isolation of apoptotic cells or removal of dead cells from cell culture or tissue preparations using annexin V magnetic beads. Annexin V is a Ca2+ dependent phospholipid binding protein with high affinity for phosphatidylserine (PS), which is redistributed from the inner to the outer plasma membrane leaflet in apoptotic or dead cells. Once on the cell surface, PS becomes available for binding to annexin V and any of its conjugates. Binding of annexin V-biotin to apoptotic cells followed by binding of the biotin to streptavidin-magnetic beads enables separation of apoptotic cells from living cells. The apoptotic cells bound to the beads adhere to the magnet, while nonapoptotic cells stay in suspension. The separated apoptotic and healthy cells can then be used in a variety of assays to study apoptotic mechanisms and pathways.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed
for 6 months from date of receipt.
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