ApoBrdu Red DNA Fragmentation Assay Kit (Fluorometric)

Product Discontinued

ApoBrdu Red DNA Fragmentation Assay Kit (Fluorometric) Summary

• Description: Convenient and reliable Br-dUTP based assay to detect DNA fragmentation by fluorescence microscopy or flow cytometry. Includes both positive and negative control cells.
  
  Detection method: Flow Cytometry (Ex/Em 480/(530/590) nm)Applications: Suitable for studying apoptosis with GFP transfected cells.
  
  
  This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab66110)
• Kit Type: Assay Kit (Fluorometric)

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Background

Internucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells. The Apo-BrdU-Red In Situ DNA Fragmentation Assay Kit provides all components including positive and negative control cells for conveniently detecting DNA fragmentation by fluorescence microscopy or flow cytometry. The kit utilizes Br-dUTP (bromolated deoxyuridine triphosphate nucleotides) which is more readily incorporated into DNA strand breaks than other larger ligands (e.g., fluorescein, biotin or digoxigenin). The greater incorporation gives rise to brighter signal when the Br-dUTP sites are identified by a Red fluorescence labeled anti-BrdU monoclonal antibody.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.