Antennapedia Control Peptide

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Immunocytochemistry/ Immunofluorescence: Antennapedia Control Peptide [NBP2-29334] - NBD inhibitory peptide but not Control peptide blocks constitutive NF-kB activation in human multiple myeloma cells. U266 cells were ...read more

Product Details

Summary
Applications ICC/IF
Concentration
LYOPH

Order Details

Antennapedia Control Peptide Summary

Description
The Antennapedia Control Peptide is composed of the protein transduction (PTD) sequence derived from antennapedia which renders the peptide cell permeable. It can be used as a control for peptides that contain the antennapedia PTD sequence.

Source: Synthetic

Amino Acid Sequence:DRQIKIWFQNRRMKWKK

Preparation
Method
Solubilize the peptide just prior to use.
Source
Synthetic
Content
Control peptide: 5 x 1 mg (lyophilized) DRQIKIWFQNRRMKWKK. Molecular weight: 2361
Protein/Peptide Type
Peptide

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence
Publications
Read Publications using
NBP2-29334 in the following applications:

  • B/N
    1 publication

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Concentration
LYOPH
Reconstitution Instructions
Researchers should determine the appropriate stock and working solutions for their model system (example for 5 mM stock solution): Add 84.8 ul of deionized water, cell culture media or DMSO to peptide. Carefully pipet to ensure peptide dissolution.

Notes

The MyD88 Homodimerization inhibitory peptide contains a protein transduction (PTD) sequence (DRQIKIWFQNRRMKWKK) derived from antennapedia which renders the peptide cell permeable (Derossi et al, The third helix of the antennapedia homeodomain translocates through biological membranes. J Biol Chem. 269:10444-10450 (1994)] .The control peptide consists of only the PTD sequence.

Alternate Names for Antennapedia Control Peptide

  • AP homeodomain Drosophilia
  • AP homeodomain

Background

Control peptide for the antennapedia-based inhibitory peptides

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.
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Publications for Antennapedia Control Peptide (NBP2-29334)(9)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: B/N.


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B/N
(1)
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Human
(1)
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(1)
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Showing Publications 1 - 9 of 9.
Publications using NBP2-29334 Applications Species
Griffiths CD, Bilawchuk LM, McDonough JE et al. IGF1R is an entry receptor for respiratory syncytial virus Nature Jun 3 2020 [PMID: 32494007] (B/N) B/N
Wu X, Xue R, Peng H et al. Traf6 inhibitor boosts antitumor immunity by impeding regulatory T cell migration in Hepa1-6 tumor model Int. Immunopharmacol. 2019 Oct 26 [PMID: 31670092] (Mouse) Mouse
Sadaka C, Marloie-Provost MA, Soumelis V, Benaroch P. Developmental regulation of MHC II expression and transport in human plasmacytoid-derived dendritic cells. Blood. 2009 Mar 5 [PMID: 19015396] (Human)

Details:
Human pDC cells were pre-incubated with IMG-2000, Fig. 7.
Human
Aggarwal S, Takada Y, Mhashilkar AM et al. Melanoma differentiation-associated gene-7/IL-24 gene enhances NF-kappa B activation and suppresses apoptosis induced by TNF. J Immunol. 2004 Oct 1 [PMID: 15383566]
Aggarwal S, Takada Y, Singh S et al. Inhibition of growth and survival of human head and neck squamous cell carcinoma cells by curcumin via modulation of nuclear factor-kappaB signaling. Int J Cancer. 2004 Sep 20 [PMID: 15252836]
Ashikawa K, Shishodia S, Fokt I et al. Evidence that activation of nuclear factor-kappaB is essential for the cytotoxic effects of doxorubicin and its analogues. Biochem Pharmacol. 2004 Jan 15 [PMID: 14698047]
Bharti AC, Takada Y, Shishodia S, Aggarwal BB. Evidence that receptor activator of nuclear factor (NF)-kappaB ligand can suppress cell proliferation and induce apoptosis through activation of a NF-kappaB-independent and TRAF6-dependent mechanism. J Biol Chem. 2004 Feb 13 [PMID: 14645259]
Bharti AC, Donato N, Aggarwal BB. Curcumin (diferuloylmethane) inhibits constitutive and IL-6-inducible STAT3 phosphorylation in human multiple myeloma cells. J Immunol. 2003 Oct 1 [PMID: 14500688]
Bharti AC, Donato N, Singh S, Aggarwal BB. Curcumin (diferuloylmethane) down-regulates the constitutive activation of nuclear factor-kappa B and IkappaBalpha kinase in human multiple myeloma cells, leading to suppression of proliferation and induction of apoptosis. Blood. 2003 Feb 1 [PMID: 12393461]

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Video Protocols

ICC/IF Video Protocol

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