Antennapedia Control Peptide

Immunocytochemistry/Immunofluorescence: Antennapedia Control Peptide [NBP2-29334] - NBD inhibitory peptide but not Control peptide blocks constitutive NF-kB activation in human multiple myeloma cells. U266 cells were treated with 100 uM of Control (A & B) or NBD peptide (C & D) for 12 hr, cytospun, plated on glass slides, air dried for 1 hr at room temperature and fixed with cold acetone. Slides were blocked with 5% normal goat serum for 1 hr and then incubated with rabbit polyclonal anti-human p65 antibody (A & C) followed by Ig-Alexa 594 second step. In control peptide treated cells, p65 translocates to nucleus (A), whereas NBD peptide prevents translocation of p65 into the nucleus (C). B & D: Nuclear staining with DNA binding dye. (Courtesy of Dr. B.B. Aggarwal, MD Anderson Cancer Center, Houston, TX)

• Applications: ICC/IF
• Concentration: LYOPH

Antennapedia Control Peptide Summary

• Description: The Antennapedia Control Peptide is composed of the protein transduction (PTD) sequence derived from antennapedia which renders the peptide cell permeable. It can be used as a control for peptides that contain the antennapedia PTD sequence.

  Source: Synthetic

  Amino Acid Sequence: DRQIKIWFQNRRMKWKK
  
  Inhibitor Content: Control peptide: 5 x 1 mg (lyophilized) DRQIKIWFQNRRMKWKK. Molecular weight: 2361
  
  Inhibitor Type: Inhibitor Peptide

• Preparation Method: Solubilize the peptide just prior to use.
• Source: Synthetic
• Content: Control peptide: 5 x 1 mg (lyophilized) DRQIKIWFQNRRMKWKK. Molecular weight: 2361
• Protein/Peptide Type: Peptide

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Concentration: LYOPH
• Reconstitution Instructions: Researchers should determine the appropriate stock and working solutions for their model system (example for 5 mM stock solution): Add 84.8 ul of deionized water, cell culture media or DMSO to peptide. Carefully pipet to ensure peptide dissolution.

Notes

The MyD88 Homodimerization inhibitory peptide contains a protein transduction (PTD) sequence (DRQIKIWFQNRRMKWKK) derived from antennapedia which renders the peptide cell permeable (Derossi et al, The third helix of the antennapedia homeodomain translocates through biological membranes. J Biol Chem. 269:10444-10450 (1994)] .The control peptide consists of only the PTD sequence.

Alternate Names for Antennapedia Control Peptide

• AP homeodomain Drosophilia
• AP homeodomain

Background

Control peptide for the antennapedia-based inhibitory peptides

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for Antennapedia Control Peptide (NBP2-29334)(14)

Publications using NBP2-29334

• Kieser QJ, Granoski MJ, McClelland RD et al. Actin cytoskeleton remodeling disrupts physical barriers to infection and presents entry receptors to respiratory syncytial virus The Journal of general virology 2023-11-01 [PMID: 38015055]
• Díaz-Dinamarca DA, Salazar ML, Escobar DF et al. Surface immunogenic protein from Streptococcus agalactiae and Fissurella latimarginata hemocyanin are TLR4 ligands and activate MyD88- and TRIF dependent signaling pathways Frontiers in immunology 2023-09-18 [PMID: 37790926]
  
  Details:
  75 µM
• Almarghlani A, Settem RP, Croft AJ et al. Interleukin-34 permits Porphyromonas gingivalis survival and NF-?B p65 inhibition in macrophages Molecular Oral Microbiology 2022-06-01 [PMID: 35576119]
• You R, Kwon OY, Woo HJ, Lee SH. Hovenia Monofloral Honey can Attenuate Enterococcus faecalis Mediated Biofilm Formation and Inflammation Food Science of Animal Resources 2022-01-01 [PMID: 35028576]
• Griffiths CD, Bilawchuk LM, McDonough JE et al. IGF1R is an entry receptor for respiratory syncytial virus Nature 2020-06-03 [PMID: 32494007] (B/N)
• Wu X, Xue R, Peng H et al. Traf6 inhibitor boosts antitumor immunity by impeding regulatory T cell migration in Hepa1-6 tumor model Int. Immunopharmacol. 2019-10-26 [PMID: 31670092] (Mouse)
• B John, C Naczki, C Patel, A Ghoneum, S Qasem, Z Salih, N Said Regulation of the bi-directional cross-talk between ovarian cancer cells and adipocytes by SPARC Oncogene, 2019-02-14;0(0):. 2019-02-14 [PMID: 30765860]
• Sadaka C, Marloie-Provost MA, Soumelis V, Benaroch P. Developmental regulation of MHC II expression and transport in human plasmacytoid-derived dendritic cells. Blood. 2009-03-05 [PMID: 19015396] (Human)
  
  Details:
  Human pDC cells were pre-incubated with IMG-2000, Fig. 7.
• Aggarwal S, Takada Y, Mhashilkar AM et al. Melanoma differentiation-associated gene-7/IL-24 gene enhances NF-kappa B activation and suppresses apoptosis induced by TNF. J Immunol. 2004-10-01 [PMID: 15383566]
• Aggarwal S, Takada Y, Singh S et al. Inhibition of growth and survival of human head and neck squamous cell carcinoma cells by curcumin via modulation of nuclear factor-kappaB signaling. Int J Cancer. 2004-09-20 [PMID: 15252836]
• Ashikawa K, Shishodia S, Fokt I et al. Evidence that activation of nuclear factor-kappaB is essential for the cytotoxic effects of doxorubicin and its analogues. Biochem Pharmacol. 2004-01-15 [PMID: 14698047]
• Bharti AC, Takada Y, Shishodia S, Aggarwal BB. Evidence that receptor activator of nuclear factor (NF)-kappaB ligand can suppress cell proliferation and induce apoptosis through activation of a NF-kappaB-independent and TRAF6-dependent mechanism. J Biol Chem. 2004-02-13 [PMID: 14645259]
• Bharti AC, Donato N, Aggarwal BB. Curcumin (diferuloylmethane) inhibits constitutive and IL-6-inducible STAT3 phosphorylation in human multiple myeloma cells. J Immunol. 2003-10-01 [PMID: 14500688]
• Bharti AC, Donato N, Singh S, Aggarwal BB. Curcumin (diferuloylmethane) down-regulates the constitutive activation of nuclear factor-kappa B and IkappaBalpha kinase in human multiple myeloma cells, leading to suppression of proliferation and induction of apoptosis. Blood. 2003-02-01 [PMID: 12393461]