Amintra (R) Ni-NTA Affinity Resin

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Amintra (R) Ni-NTA Affinity Resin Summary

Description
Amintra (R) Ni-NTA affinity resin is designed for simple, rapid His-tagged recombinant protein purification from a cell lysate under native or denaturing conditions. Metal chelate affinity chromatography is a rapid one-step purification, which removes most contaminants and can achieve purities close to homogeneity.

Amintra(R) Ni-NTA affinity resin offers excellent flow properties and high protein binding capacity: in excess of 50mg protein/ml.

NTA is a tetradentate chelator which occupies four of the six binding sites in the coordination sphere of the nickel ion. The other two coordination sites are usually occupied by water molecules and can interact with histidine residues of the recombinant protein. This binding minimizes metal leaching during purification.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.

Notes

This product is manufactured by Expedeon Inc. and distributed by Novus Biologicals.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

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