Human Alzheimer Associated Neuronal Thread Protein ELISA Kit (Colorimetric)

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ELISA: Human Alzheimer Associated Neuronal Thread Protein ELISA Kit (Colorimetric) [NBP2-59879]
Human Alzheimer Associated Neuronal Thread Protein ELISA Kit (Colorimetric) [NBP2-59879] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and tissue homogenates
Standard Curve Range
93.75 pg/mL - 6000 pg/mL
Sensitivity
23.43 pg/mL

Order Details

Human Alzheimer Associated Neuronal Thread Protein ELISA Kit (Colorimetric) Summary

Description
Assay Length: 1-5 hours
Specificity
This assay has high sensitivity and excellent specificity for detection of human AD7C-NTP. No significant cross-reactivity or interference between human AD7C-NTP and analogues was observed.
Standard Curve Range
93.75 pg/mL - 6000 pg/mL
Sensitivity
23.43 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 10
Intra-Assay
%CV < 8
Sample Volume
50 - 100 uL
Kit Type
ELISA Kit (Colorimetric)

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Adhesive Strip (For 96 wells)
  2. Assay plate (12 x 8 coated Microwells)
  3. Biotin-antibody (100 x concentrate)
  4. Biotin-antibody Diluent
  5. HRP-avidin (100 x concentrate)
  6. HRP-avidin Diluent
  7. Instruction manual
  8. Sample Diluent
  9. Standard (Freeze dried)
  10. Stop Solution
  11. TMB Substrate
  12. Wash Buffer (25 x concentrate)

Alternate Names for Human Alzheimer Associated Neuronal Thread Protein ELISA Kit (Colorimetric)

  • AD7C-NTP
  • Alzheimer Associated Protein
  • Alzheimer NTP
  • Neural Thread Protein
  • Neuronal Thread Protein

Background

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for AD7C-NTP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AD7C-NTP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AD7C-NTP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AD7C-NTP bound in the initial step. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for Alzheimer Associated Neuronal Thread Protein ELISA Kit (NBP2-59879). (Showing 1 - 1 of 1 FAQ).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

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