Alkaline Phosphatase Assay Kit (Colorimetric)

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Alkaline Phosphatase Assay Kit (Colorimetric) [KA0817] - The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay ...read more

Product Details

Summary
Reactivity Hu, Mu, MaSpecies Glossary
Applications Func, Quantification
Suitable Sample Type
Biological Fluid, Cell Lysate, Culture Medium, Plasma, Serum, Tissue Lysate, Urine
Standard Curve Range
10 to 250 uU

Order Details

Alkaline Phosphatase Assay Kit (Colorimetric) Summary

Description
Alkaline Phosphatase Assay Kit is used for detecting ALP activity.
Specificity
This kit can detect all forms of alkaline phosphatase (intestinal, placental, germ cell, liver/bone/kidney).
Standard Curve Range
10 to 250 uU
Assay Type
Colorimetric
Sample Volume
80 uL
Kit Type
Assay Kit (Colorimetric)

Applications/Dilutions

Application Notes
Contains 10 substrate tablets providing convenience for multiple usages. The kit uses p-nitrophenyl phosphate (pNPP) as a phosphatase substrate which turns yellow (max= 405 nm) when dephosphorylated by ALP. The kit can detect 10-250 uU ALP in samples.
Publications
Read Publication using KA0817.

Packaging, Storage & Formulations

Storage
Store at -20C.

Kit Components

Components
  1. Stop Solution
  2. ALP Assay Buffer
  3. ALP Enzyme
  4. pNPP (10 TAB)

Notes

Quality control test: Standard curve

This product is produced by and distributed for Abnova, a company based in Taiwan.

Background

Alkaline phosphatase (ALP) catalyzes the hydrolysis of phosphate esters in alkaline buffer and produces an organic radical and inorganic phosphate. Changes in alkaline phosphatase level and activity are associated with various disease states in the liver and bone.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Publications for Alkaline Phosphatase Kit (KA0817)(1)

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