Alkaline Phosphatase, Bacterial Antibody (L2)

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Product Details

Summary
Reactivity V-ViSpecies Glossary
Applications WB, ICC/IF, IP
Clone
L2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Alkaline Phosphatase, Bacterial Antibody (L2) Summary

Immunogen
F201C1B.
Specificity
Alkaline phosphatase (AP). MAB1012 has a high affinity and recognizes an AP determinant resistant to denaturation by SDS-PAGE. It is therefore ideally suited for sensitive and specific detection of AP fusion proteins by Western Blot analysis of E. coli transformants expressing fusion products.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
phoA
Purity
Unpurified
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Applications/Dilutions

Dilutions
  • Western Blot 1:5000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 1:10-1:500
Application Notes
Western Blot at 42-45kDa on SDS-PAGE,reducing gels for natural E. coli alkaline phosphatase monomer. Alkaline phosphatase fusion proteins will vary depending upon the target fusion protein. Immunocytochemistry/Immunofluorescence : reacts with E.coli. AP fusion protein targets in acetone fixed cell preparations. 1:4000, other fixatives or conditions untested. ASSAY: Preparation of E. coli TnphoA transformants: E. coli strain CC118 was transformed with plasmid pGEM-3Z containing TnphoA insertional mutations in the p101 gene of Mycoplasma hyorhinis, which encodes a protein with a typical N-terminal prokaryotic single peptide (Yogev et al. 1991). Identification of fusion protein with MAB1012 transformants: Transformants are grown in 2XYT medium to OD600=0.6. Cells were centrifuged 3 minutes at 10,000 x g, suspended in SDS-PAGE sample buffer, heated at 100C for 5 minutes, frozen and thawed and centrifuged as above at room temperature to remove insoluble material. The sample is applied at 9% to a SDS-PAGE gel, and Western immunoBlot is performed as described (Yogev et al. 1991). Immunoprecipitation: 5ul of antibody per 500ul of lysate in RIPA or 0.5% triton X-100 solutions.

Reactivity Notes

E. coli

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
No Preservative
Purity
Unpurified

Notes

Relevant Entrez Gene Numbers: 945041

Alternate Names for Alkaline Phosphatase, Bacterial Antibody (L2)

  • BAP
  • ECK0378
  • JW0374
  • Pho A
  • PhoA

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol phoA
Uniprot