Aldosterone ELISA Kit (Colorimetric)

ELISA: Aldosterone ELISA Kit (Colorimetric) [KA1883] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

• Applications: ELISA, NULL
• Suitable Sample Type: Plasma, Serum, Urine
• Standard Curve Range: 20 to 1000 pg/mL
• Sensitivity: 10 pg/mL

Aldosterone ELISA Kit (Colorimetric) Summary

• Description: Aldosterone ELISA Kit is an enzyme immunoassay for the quantitative determination of Aldosterone in human serum, plasma, and urine.
• Standard Curve Range: 20 to 1000 pg/mL
• Sensitivity: 10 pg/mL
• Assay Type: Quantification
• Inter-Assay: CV% < 9.57%
• Intra-Assay: CV% < 6.63%
• Spike Recovery: 1.0649
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA
  • Quantification

Packaging, Storage & Formulations

• Storage: Store at 4C.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Aldosterone ELISA Kit (Colorimetric)

• Aldosterone

Background

Aldosterone is a steroid hormone (mineralocorticoid family) produced by the outer-section (zona glomerulosa) of the adrenal cortex in the adrenal gland to regulate sodium and potassium balance in the blood. It is synthesized from cholesterol by aldosterone synthase, which is absent in other sections of the adrenal gland. It acts in the kidney promoting the reabsorption of sodium ions (Na+) into the blood. Water follows the salt and this helps maintain normal blood pressure. It also acts on sweat glands to reduce the loss of sodium in perspiration and taste cells to increase the sensitivity of the taste buds to sources of sodium.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Aldosterone ELISA Kit (KA1883)(3)

Publications using KA1883

• Gretchen S, Katharina G, Ali J et al. Involvement of circulating factors in the transmission of paternal experiences through the germline. EMBO J. 2020-10-09 [PMID: 33034389]
• Schaefer J, Burckhardt BB, Tins J et al. Validated low-volume aldosterone immunoassay tailored to GCLP-compliant investigations in small sample volumes. Pract Lab Med 2017-07-27 [PMID: 29034304]
• Cannavo A, Liccardo D, Eguchi A et al. Myocardial pathology induced by aldosterone is dependent on non-canonical activities of G protein-coupled receptor kinases. Nat Commun 2016-03-02 [PMID: 26932512] (WB)

FAQs for Aldosterone ELISA Kit (KA1883). (Showing 1 - 2 of 2 FAQs).

1. Could this kit be used on rat plasma? Or only human?
   • I believe this kit should work with rat plasma since the target is not species specific, as long as aldosterone is present in the sample.
2. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.