Agarose Streptavidin


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Agarose Streptavidin Summary

  • Prepared by conjugating streptavidin to heat stable, cross-linked 4% agarose gel beads.
  • To ensure minimal steric interference and low nonspecific binding, streptavidin is conjugated through a hydrophilic spacer arm.
  • Biotin binding activity of the streptavidin optimally preserved through proprietary coupling procedure.
  • Can be used to separate biotinylated macromolecules from unbiotinylated materials or for solid phase binding assays.
  • Streptavidin cannot be leached from gel with solutes such as Tris buffer.
  • Low non-specific binding activity of gel.
  • Protein concentration is 1 mg streptavidin per ml of settled agarose beads.
  • Supplied as a 1:1 suspension in buffer.
  • Binding capacity is approximately 1 mole biotinylated HRP per mole streptavidin.
  • Applications/Dilutions

    Application Notes
    Binding capacity: >1 mole of biotinylated horseradish peroxidase/mole of streptavidin

    Packaging, Storage & Formulations

    Store at 4C. Do not freeze.
    Suspension solution: 10 mM HEPES, pH 8.0, 0.15 M NaCl
    0.08% Sodium Azide


    This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.


    This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

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