Adenylate Kinase 6 Antibody (B1-F4)

Western Blot: Adenylate Kinase 6 Antibody (B1-F4) [NBP2-67116] - Analysis of AK6 on MCF-7 cell (1) and human lung tissue (2) lysate using anti-AK6 antibody at 1/1000 dilution.

Immunocytochemistry/Immunofluorescence: Adenylate Kinase 6 Antibody (B1-F4) [NBP2-67116] - Staining AK6 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Flow Cytometry: Adenylate Kinase 6 Antibody (B1-F4) [NBP2-67116] - Analysis of MCF-7 cells with AK6 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

Product Discontinued

Adenylate Kinase 6 Antibody (B1-F4) Summary

• Immunogen: Synthetic peptide within Human Adenylate Kinase 6 aa 123-172 / 172. (SwissProt: Q9Y3D8 Human)
• Localization: Nucleus.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: AK6
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:00
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Western Blot 1:2000-1:5000

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 2 mg/ml
• Purity: Protein A purified

Alternate Names for Adenylate Kinase 6 Antibody (B1-F4)

• AD-004
• Adenylate Kinase 6
• adenylate kinase isoenzyme 6
• adrenal gland protein AD-004
• AK/ATPase
• AK6
• ATP-AMP transphosphorylase 6
• CGI-137
• CINAP
• CIP
• coilin interacting nuclear ATPase protein
• coilin interacting protein
• Coilin-interacting nuclear ATPase protein
• Dual activity adenylate kinase/ATPase
• EC=2.7.4.3
• hCINAP

Background

This gene encodes a protein that belongs to the adenylate kinase family of enzymes. The protein has a nuclear localization and contains Walker A (P-loop) and Walker B motifs and a metal-coordinating residue. The protein may be involved in regulation of Cajal body formation. In human, AK6 and TAF9 (GeneID: 6880) are two distinct genes that share 5' exons. Alternative splicing results in multiple transcript variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AK6