ADCK5 Antibody

Western Blot: ADCK5 Antibody [NBP1-83500] - Analysis in control (vector only transfected HEK293T lysate) and ADCK5 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Immunocytochemistry/Immunofluorescence: ADCK5 Antibody [NBP1-83500] - Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.

Immunohistochemistry-Paraffin: ADCK5 Antibody [NBP1-83500] - Staining of human pancreas shows strong cytoplasmic positivity in islet cells.

Product Discontinued

ADCK5 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: VLLDHGLYQFLEEKDRAALCQLWRAIILRDDAAMRAHAAALGVQDYLLFAEMLMQRPVRLGQLWGSHLLSREEAAYMVDMARERFEAVMAVLRELP
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ADCK5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Theoretical MW: 66 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (81%), Rat (82%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for ADCK5 Antibody

• aarF domain containing kinase 5
• EC 2.7.11.-
• FLJ35454
• MGC126708

Background

The function of this protein is not yet clear. It is not known if it has protein kinase activity and whattype of substrate it would phosphorylate (Ser, Thr or Tyr)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ADCK5
• Entrez:
  • 203054(Human)
• Uniprot:
  • Q3MIX3(Human)