Active Caspase Apoptosis Detection Kit

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Product Details

Summary
Reactivity Hu, Mu, Rt, MaSpecies Glossary
Applications Flow
Suitable Sample Type
Live cells

Order Details

Active Caspase Apoptosis Detection Kit Summary

Description
Simple and convenient kit for detecting activation of Caspases by Flow Cytometry in living cells.
  • Detection method: Flow cytometry L-1 channel (Ex/Em = 488/530 nm).
  • Applications: Detection of activated caspases in living cells.
Kit Type
Apoptosis Detection Kit

Applications/Dilutions

Dilutions
  • Flow Cytometry

Packaging, Storage & Formulations

Storage
Store at -20C.

Kit Components

Components
  1. D2R Incubation Buffer
  2. D2R Reagent
  3. DTT (1 M)

Background

Activation of members of caspase-family proteases plays a key role in apoptosis. The Apoptosis Detection Kit provides a convenient means for detecting activation of caspases by flow cytometry in living cells. The assay is based on the cleavage of (aspartyl)2-Rhodamine 110 (D2R), a reported substrate for members of caspase family proteases. The caspase substrate D2R is non-fluorescent, however, upon cleavage of the substrate by cellular caspases, the released rhodamine 110 gives rise to fluorescence that can be measured at excitation of 488 nm and emission of 530 nm.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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