Active Caspase Staining Kit (Fluorometric) Summary
| Description |
Convenient kit to detect activated caspases in living cells. Detection Method: Fluorescence Microscopy, Flow Cytometry or Fluorescence Plate Reader. Detection method: Flow cytometry using FL2 channel Ex/Em = 540/570 nm.Applications: Detection of activated caspases in living cells. This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab65616) |
| Kit Type |
Staining Kit (Fluorometric) |
Applications/Dilutions
Packaging, Storage & Formulations
Kit Components
|
Components
|
- Red-VAD-FMK
- Wash Buffer
- Z-VAD-FMK
|
Background
Activation of caspases plays a central role in apoptosis. The Caspase Staining Kit provides a convenient means for detecting activated caspases in living cells. The assay utilizes a caspase family inhibitor VAD-FMK conjugated to sulfo-rhodamine (Red-VAD-FMK) as the fluorescent marker. Red-VAD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspases in apoptotic cells.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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