Active Caspase Assay Kit (Fluorometric) Summary
| Description |
Convenient and sensitive kit to detect activated caspases in living cells. Detection method: Fluorescence microscopy, plate reader (Ex/Em = 540/570 nm) and Flow cytometryApplications: Detection of activated caspases in living cells. This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab65611) |
| Kit Type |
Assay Kit (Fluorometric) |
Applications/Dilutions
Packaging, Storage & Formulations
Kit Components
|
Components
|
- FITC-VAD-FMK
- Wash Buffer
- Z-VAD-FMK
|
Background
Activation of caspases plays a central role in apoptosis. The Active Caspase Staining Kit provides a convenient and sensitive means for detecting activated caspases in living cells. The assay utilizes the caspase family inhibitor VAD-FMK conjugated to FITC (FITC-VAD-FMK) as a marker. FITC-VAD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspases in apoptotic cells.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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