Active Beta Catenin Pathway Assay Kit Summary
Description |
Active beta-Catenin qualitative ELISA has been designed to detect the active dephosphorylated form of beta-Catenin in many types of samples subjected to different experimental conditions. It is particularly suitable for monitoring the activation of Wnt signaling on the levels of active beta-Catenin. |
Specificity |
This kit contains (1) 96 well strip plate containing Active Beta-Catenin (dephosphorylated) (DP S33/S37/T41) 6 pairs of strips (12 strips total) with a kit assay control useful for detecting the dephosphorylated form of Beta-Catenin which is the active form.Dephosphorylated- (DP S33/S37/T41) |
Kit Type |
Pathway Assay Kit |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Store at 4C. Do not freeze. |
Kit Components
Components
|
- 2 x 8-microwell strips
- 20x Wash buffer Concentrate
- 5x Sample Dilution Buffer Concentrate
- Antibody-Coated Microwells
- Assay Control
- Biotin-Conjugated Detection Antibody
- Microwell Strip Holder
- Plate Cover
- Stop Solution
- Streptavidin Horseradish Peroxidase
- Tetramethylbenzidine Substrate
|
Notes
You may also Mix-and-Match you own Pathway Assay Kit by purchasing catalog number NBP1-71668. Choose up to 6 different targets from this list: Active Beta Catenin (DP S33/S37/T41), AKT1 (pS473), AKT2 (pS474), CREB (pS133), ERK1/2 (pT202/Y204; pT185/Y187), GSK3 alpha (pS21), GSK3 beta (pS9), p38 (pT180/Y182).
View All Available Pathway Assay Kits
Background
Multi-kinase arrays are an alternative analysis platform to traditional Western blots. Novus' Pathway Assay Kits allow for the simultaneous detection of activation states of multiple kinases in a signal pathway on a single multiwell platform. These kits are easier and faster than traditional Western blots (2.5 hour protocol), demonstrate a significantly greater dynamic range and sensitivity, operate with much less sample (5-20 micrograms total protein), and are more cost-effective with up to six data points per assay.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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