ACSM5 Overexpression Lysate (Native)


Western Blot: ACSM5 Overexpression Lysate (Adult Normal) [NBL1-07269] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ACSM5.

Product Details

Applications WB
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Order Details

ACSM5 Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC209798 and based on accession number NM_017888. The protein contains a C-terminal DDK tag.
C-terminal Myc/DDK
Homo sapiens acyl-CoA synthetase medium-chain family member 5 (ACSM5), mRNA.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.


Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
64.6 kDa

Lysate Details for ACSM5

Protein State


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for ACSM5 Overexpression Lysate (Native)

  • acyl-CoA synthetase medium-chain family member 5
  • acyl-coenzyme A synthetase ACSM5, mitochondrial
  • EC 6.2.1
  • EC
  • FLJ20581
  • MACS3


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Video Protocols

WB Video Protocol

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Additional ACSM5 Overexpression Lysate (Native) Products

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Contact Information

Product PDFs


Gene Symbol ACSM5

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