Acetylcholine ELISA Kit (Colorimetric)

ELISA: Acetylcholine ELISA Kit (Colorimetric) [NBP2-66389] - Samples were spiked with high concentrations of Acetylcholine and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Acetylcholine ELISA Kit (Colorimetric) [NBP2-66389] - Standard Curve Reference

• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 15.63 - 1000 pg/mL
• Sensitivity: 9.38 pg/mL

Acetylcholine ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes ACH in samples. No significant cross-reactivity or interference between ACH and analogues was observed.
• Standard Curve Range: 15.63 - 1000 pg/mL
• Sensitivity: 9.38 pg/mL
• Assay Type: Competitive-ELISA
• Inter-Assay: CV% < 4.94%
• Intra-Assay: CV% < 4.37%
• Spike Recovery: 87-102%
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Acetylcholine ELISA Kit (Colorimetric)

• [2 (acetyloxy)ethyl]trimethylammonium
• ACh

Background

The chemical compound Acetylcholine, often abbreviated as ACh, was the first neurotransmitter to be identified. It is a chemical transmitter in the central nervous system (CNS) as well as in the parasympathetic nervous system in many organisms including humans. When it binds to Acetylcholine receptors of muscle fibers, stimulates those fibers to contract. Acetylcholine is also used in the brain, where it tends to cause excitatory actions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Acetylcholine ELISA Kit (NBP2-66389)(5)

Publications using NBP2-66389

• Kirkland, JM;Patel, I;Ardeshna, MS;Kopec, AM; Microglial synaptic pruning in the nucleus accumbens during adolescence sex-specifically influences splenic immune outcomes bioRxiv : the preprint server for biology 2023-05-05 [PMID: 37205376] (ELISA)
• Hwang J, Okada J, Liu L et al. Ablation of parasympathetic cholinergic innervation of the liver prevents diet-induced obesity and hepatic steatosis in mice bioRxiv 2023-11-05 (ELISA)
• Hwang J, Okada J, Liu L et al. The development of hepatic steatosis depends on the presence of liver-innervating parasympathetic cholinergic neurons in mice fed a high-fat diet. PLoS biology 2024-11-01 [PMID: 39436946]
• Naz F, Sajid S, Yueda L et al. A comparative study of anti-aging effects of Carica Papaya (pulp and seeds) on D-galactose induced brain aging in albino rats Journal of Clinical and Translational Research 2022-09-28 [PMID: 36451797]
• Vang A, da Silva GonCalves Bos D, Fernandez-Nicolas A et al. Alpha 7 nicotinic acetylcholine receptor mediates right ventricular fibrosis and diastolic dysfunction in pulmonary hypertension JCI insight 2021-05-11 [PMID: 33974567] (ELISA, ELISA)

FAQs for Acetylcholine ELISA Kit (NBP2-66389). (Showing 1 - 2 of 2 FAQs).

1. Are there components containing recombinant protein in these kits NBP2-66389 and NBP2-62171?
   • There are no recombinant proteins in this ELISA kits.
2. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.