AbSelect™ TCS Purification System Antibody Purification Kit

Images

 
There are currently no images for AbSelect (TM) TCS Purification System Kit (862-0030).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary

Order Details

AbSelect™ TCS Purification System Antibody Purification Kit Summary

Description
Antibodies are often generated from hybridoma cell lines and supplied in tissue culture supernatant (TCS). TCS itself contains many substances that interfere with antibody labeling reactions. Successful labeling therefore requires antibodies to be purified prior to labeling. The AbSelect TCS system is a fast and simple method to purify these antibodies.

AbSelect TCS is prepared by coupling highly purified protein A to agarose beads which can be used to purify IgG fractions from hybridoma supernatants. The method involves the capture of the antibody on the AbSelect TCS resin and the removal of unwanted substances using a simple wash procedure. The purified product is then eluted and neutralized.

The components of the AbSelect TCS are fully compatible with the Lightning-Link antibody labeling kits (available separately), which allows the purified antibody to be immediately labeled with an enzyme, fluorescent protein or fluorescent dye, with a hands-on time of under 30 seconds.

Benefits:
  • All components and buffers supplied
  • Purify antibody from 10 - 50 mls of hybridoma TCS
Kit Type
Antibody Purification Kit

Applications/Dilutions

Application Notes
The antibody to be purified should be in 10 to 50 mL of tissue culture supernatant. Up to 5 mg of antibody can be purified in each run.

Packaging, Storage & Formulations

Storage
Store at 4C.

Kit Components

Components
  1. AbSelect™ TCS Protein A Resin (1 vial)
  2. Binding buffer - 10X (1 bottle)
  3. Concentrator spin column and collection tube (1 EA)
  4. Elution buffer (1 bottle)
  5. Neutralization buffer (1 vial)
  6. Purification column (1 EA)
  7. Wash buffer (1 bottle)

Notes

AbSelect® is a registered trademark of Expedeon Inc. and distributed by Novus Biologicals.

Background

Antibodies are often generated from hybridoma cell lines and supplied in tissue culture supernatant (TCS). TCS itself contains many substances that interfere with antibody labeling reactions. Successful labeling therefore requires antibodies to be purified prior to labeling. The AbSelect TCS system is a fast and simple method to purify these antibodies. The components of the AbSelect TCS are fully compatible with the Lightning-Link antibody labeling kits (available separately), which allows the purified antibody to be immediately labeled with an enzyme, fluorescent protein or fluorescent dye, with a hands-on time of under 30 seconds.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Publications for AbSelect (TM) TCS Purification System Kit (862-0030) (0)

There are no publications for AbSelect (TM) TCS Purification System Kit (862-0030).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for AbSelect (TM) TCS Purification System Kit (862-0030) (0)

There are no reviews for AbSelect (TM) TCS Purification System Kit (862-0030). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for AbSelect (TM) TCS Purification System Kit (862-0030) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

Additional AbSelect (TM) TCS Purification System Products

Blogs on AbSelect (TM) TCS Purification System

There are no specific blogs for AbSelect (TM) TCS Purification System, but you can read our latest blog posts.
Coronavirus Brochure

Contact Information

Product PDFs

Review this Product

Be the first to review our AbSelect (TM) TCS Purification System Antibody Purification Kit and receive a gift card or discount.