AbSelect™ Rat TCS Antibody Purification Kit

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AbSelect™ Rat TCS Antibody Purification Kit

Product Details

Summary

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AbSelect™ Rat TCS Antibody Purification Kit Summary

Description
The AbSelect™ Rat TCS Antibody Purification System enables the selective purification of rat IgG from hybridoma supernatants without the co-purification of bovine IgG. This is because AbSelect™ Rat resin has a high affinity and specificity for rat IgG molecules whereas the binding strength of bovine IgG to AbSelect™ Rat resin is negligible. In comparison, Protein G does not have the capability to selectively purify rat IgG from hybridoma supernatants.

The method of AbSelect™ Rat TCS Antibody Purification System involves capture of the antibody on the AbSelect™ Rat resin and the removal of unwanted substances using a simple wash procedure. The purified antibody is then eluted and neutralized. AbSelect™ Rat TCS Antibody Purification System is not suitable for use with antibodies from other species.

The components of all AbSelect™ kits are fully compatible with the Lightning-Link® and Lightning-Link® antibody labeling systems (available separately), which allow the purified antibody to be rapidly conjugated to a wide variety of labels.
Kit Type
Antibody Purification Kit

Applications/Dilutions

Application Notes
The rat antibody to be purified should be in 10 to 50ml of tissue culture supernatant. Up to 600ug of antibody can be purified in each run.

Packaging, Storage & Formulations

Storage
Store at 4C.

Kit Components

Components
  1. 1 vial of Elution Buffer
  2. 1 vial of 10x Binding Buffer
  3. 1 vial of Neutralization Buffer
  4. 1 vial of Wash Buffer
  5. 12 additional collecting tubes
  6. 3 vials of AbSelectTM Rat resin
  7. 3 spin cartridge/ collecting tube assemblies
  8. 3 concentrator spin columns

Notes

AbSelect® is a registered trademark of Expedeon Inc. and distributed by Novus Biologicals.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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