AbSelect™ Generic Antibody Purification Kit Summary
Commercially available antibodies often contain substances (e.g. BSA, glycine, tris, azide) that interfere in labeling reactions. The AbSelect Purification Kit quickly removes these contaminants. It can also be used to purify antibodies from crude samples such as ascites fluid or immune serum.
The antibody to be purified or cleaned up ideally is in a volume of 100 ul to 0.5 ml. Up to 500 ug of antibody can be purified in each run. The AbSelect method involves capture of the antibody on protein A resin and the removal of unwanted substances by a simple wash procedure. The purified product is then eluted and neutralized.
The components of the AbSelect kit are fully compatible with the Lightning-Link antibody labeling system (available separately), which allows the purified antibody to be rapidly conjugated with a wide variety of labels.
Antibody Purification Kit
The volume of antibody to be purified or cleaned up should be 0.1-0.5ml, though larger volumes may be processed by first incubating the antibody sample (combined with the Binding Buffer) with the Protein A resin in a larger vessel (e.g. 2ml eppendorf) prior to transferring to the spin cartridge in several aliquots, spinning down the excess liquid each time.
Packaging, Storage & Formulations
- 1 vial of 10x Binding buffer
- 1 vial of Elution buffer
- 1 vial of Neutralization buffer
- 1 vial of Washing buffer
- 1 or 3 spin cartridge / collecting tube assemblies
- 1 or 3 vials of AbSelect Protein A resin
- 4 or 12 additional collecting tubes
AbSelect® is a registered trademark of Expedeon Inc. and distributed by Novus Biologicals.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed
for 6 months from date of receipt.
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