Commercially available antibodies often contain substances (e.g. BSA, glycine, tris, azide) that interfere in labeling reactions. The AbSelect Purification Kit quickly removes these contaminants. It can also be used to purify antibodies from crude samples such as ascites fluid or immune serum.
The antibody to be purified or cleaned up ideally is in a volume of 100 ul to 0.5 ml. Up to 500 ug of antibody can be purified in each run. The AbSelect method involves capture of the antibody on protein A resin and the removal of unwanted substances by a simple wash procedure. The purified product is then eluted and neutralized.
The components of the AbSelect kit are fully compatible with the Lightning-Link antibody labeling system (available separately), which allows the purified antibody to be rapidly conjugated with a wide variety of labels.
Antibody Purification Kit
The volume of antibody to be purified or cleaned up should be 0.1-0.5ml, though larger volumes may be processed by first incubating the antibody sample (combined with the Binding Buffer) with the Protein A resin in a larger vessel (e.g. 2ml eppendorf) prior to transferring to the spin cartridge in several aliquots, spinning down the excess liquid each time.
Read 1 Review rated 5 using 860-0005 in the following application:
1 or 3 spin cartridge / collecting tube assemblies
1 vial of Elution buffer
1 vial of 10x Binding buffer
1 or 3 vials of AbSelect Protein A resin
1 vial of Washing buffer
1 vial of Neutralization buffer
4 or 12 additional collecting tubes
AbSelect® is a registered trademark of Expedeon Inc. and distributed by Novus Biologicals.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.
Publications for AbSelect (TM) Generic Kit (860-0005) (0)
There are no publications for AbSelect (TM) Generic Kit (860-0005).
By submitting your publication information earn gift cards and discounts for future purchases.
Review for AbSelect (TM) Generic Kit (860-0005) (1)
Average Rating: 5 (Based on 1 review)
We have 1 review tested in 1 species: Human.
Reviews using 860-0005:
Filter by Applications
Filter by Species
reviewed by: Alissa Taylor
commerically available IFN antibody
FAQs for AbSelect (TM) Generic Kit (860-0005). (Showing 1 - 2 of 2 FAQ).
Can AbSelect Antibody Purification Kits be used for purification of other proteins in addition to antibodies?
No, the AbSelect Antibody Purification Kit is not suitable for purifying proteins other than antibodies. This kit uses a Protein A resin. Protein A binds to antibodies but not other proteins. The antibody molecules are therefore bound to the resin, while any other proteins in the sample are simply washed through in the wash step, and hence are removed. The antibody is then detached from the resin in the elution step. Therefore, you should not attempt to purify anything other than antibodies using an AbSelect kit. However, you may be interested in one of our Antibody Concentration and Clean Up Kits, catalog numbers 861-0010 (3 columns) and 861-0005 (1 column). This product, in contrast to AbSelect, purifies by size: the sample is passed through a filter with a 10 kDa molecular weight cut-off. Therefore, if your protein is not too small, and you only want to remove low molecular weight substances, you can use the Antibody Concentration and Clean Up Kit.
I am looking to purchase NB110-8392, but need a formulation without Sodium Azide. Would a product like this be available?
I have searched our product database for an IL3 antibody without sodium azide but was not successful. My recommendation would be to go ahead and purchase the antibody you were originally looking at, NB110-8392, and using our AbSelect Antibody Purification Kit, cat#860-0005 to clean it up. This kit is designed for removing substances such as azide, BSA, glycine and tris.
PRODUCT AVAILABILITY: Update Regarding the Evolving COVID-19 Situation
Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.