Description | Commercially available antibodies often contain substances (e.g. BSA, glycine, tris, azide) that interfere in labeling reactions. The AbSelect Purification Kit quickly removes these contaminants. It can also be used to purify antibodies from crude samples such as ascites fluid or immune serum. The antibody to be purified or cleaned up ideally is in a volume of 100 ul to 0.5 ml. Up to 500 ug of antibody can be purified in each run. The AbSelect method involves capture of the antibody on protein A resin and the removal of unwanted substances by a simple wash procedure. The purified product is then eluted and neutralized. The components of the AbSelect kit are fully compatible with the Lightning-Link antibody labeling system (available separately), which allows the purified antibody to be rapidly conjugated with a wide variety of labels. |
Kit Type | Antibody Purification Kit |
Application Notes | The volume of antibody to be purified or cleaned up should be 0.1-0.5 mL, though larger volumes may be processed by first incubating the antibody sample (combined with the Binding Buffer) with the Protein A resin in a larger vessel (e.g. 2 mL-eppendorf tube) prior to transferring to the spin cartridge in several aliquots, spinning down the excess liquid each time. |
|
Reviewed Applications |
|
Storage | Store at 4C. |
Components |
|
Images | Ratings | Applications | Species | Date | Details | ||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
reviewed by:
Alissa Taylor |
ELISA | Human | 09/24/2014 |
Summary
|
|||||||||
reviewed by:
amy chamberlain |
ELISA | 25046 | 09/15/2011 |
Summary
|