| Description | Commercially available antibodies often contain substances (e.g. BSA, glycine, tris and azide) that interfere in labeling reactions with enzymes, biotin, streptavidin or fluorophores. The AbSelect G Purification System quickly removes these contaminants. It can also be used to purify antibodies from crude samples such as ascites fluid or immune serum. The antibody to be purified or cleaned up ideally is in a volume of 0.1 ml to 0.5 ml. Up to 500 ug of antibody can be purified in each run. The method involves capturing the antibody on the AbSelect Protein G resin. Protein G has a high affinity for the Fc regions of IgG molecules from a variety of species. Once the antibody has bound to the Protein G, unwanted substances can be removed by simply washing the resin. The purified product is then eluted and neutralized. The bSelect G Purification System is fully compatible with both the Lightning-Link and Lightning-Link Rapid conjugation systems (available separately), which allow the purified antibody to be immediately labeled with a hands-on time of under 30 seconds. Benefits:
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| Kit Type | Antibody Purification Kit |
| Application Notes | The volume of antibody to be purified or cleaned up should be 0.1-0.5ml, though larger volumes may be processed by first incubating the antibody sample (combined with Binding Buffer) with the Protein G resin in a larger vessel (e.g. 2ml eppendorf) prior to transferring to the spin cartridge in several aliquots, spinning down the excess liquid each time. |
| Storage | Store at 4C. |
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