Abpure™ TCS Antibody Purification Kit

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Summary

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Abpure™ TCS Antibody Purification Kit Summary

Description
Protein A has a high affinity for the Fc regions of IgG molecules from a variety of species. AbPure Protein A resin is prepared by coupling purified Protein A to agarose beads. It can therefore be used to purify IgG fractions from hybridoma supernatants.

The method involves capture of the antibody on the AbPure resin and the removal of unwanted substances using a simple wash procedure. The antibody is then eluted and neutralized. The kit can be used in its rapid version by spin-purification in a centrifuge, or by gravity-flow purification. The AbPure TCS System is fully compatible with the InnovaCoat GOLD and the LATEX conjugation kits (available separately), which allow the purified antibody to be conjugated rapidly.
Kit Type
Antibody Purification Kit

Applications/Dilutions

Application Notes
The antibody to be purified should be in 10 to 50 ml of tissue culture supernatant (TCS). Up to 5 mg of antibody can be purified in each run.

Packaging, Storage & Formulations

Storage
Store at 4°C.

Kit Components

Components
  1. 1 bottle of 10x AbPure Binding Buffer
  2. 1 bottle of AbPure Elution Buffer
  3. 1 bottle of AbPure Neutralization Buffer
  4. 1 bottle of AbPure Preservative
  5. 1 bottle of AbPure Wash Buffer
  6. 1 or 3 bottles of AbPure TCS Protein A resin
  7. 1 or 3 concentrator spin columns
  8. 1 or 3 spin purification columns with caps and stoppers
  9. 4 or 12 15ml collection tubes

Notes

This prodcut is manufactured by Abcam and distributed by Novus Biologicals.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt and this statement overrides any mentioned guarantee period on the limitations section of this products datasheet. Please contact technical@novusbio.com with questions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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