ABpure™ Antibody Purification Kit

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ABpure Antibody Purification Kit [260-0010] - SDS-PAGE shows the sample containing Rabbit IgG pre and post purification by using AbPure Purification kit. BSA has been successfully removed without compromising the ...read more

Product Details

Summary

Order Details

ABpure™ Antibody Purification Kit Summary

Description
Commercially available antibodies often contain substances (e.g. BSA and other proteins, glycine, tris, and azide) that interfere in conjugation reactions. The AbPure Antibody Purification System quickly removes these contaminants. It can also be used to purify antibodies from crude samples such as ascites fluid or immune serum. The antibody to be purified or cleaned up ideally is in a volume of 0.1ml to 0.5ml. 20 to 500ug of antibody can be purified in each run.

Binding Affinity
Species Isotype Binding Strength
Rabbit IgG High
Human IgG High
Pig IgG High
Mouse IgG1 Low/Medium
Mouse IgG2a High
Mouse IgG2b High
Mouse IgG3 Low/Medium
Goat IgG Low
Sheep IgG Low
Rat IgG Low
Kit Type
Antibody Purification Kit

Applications/Dilutions

Application Notes
The volume of antibody to be purified or cleaned up should be 0.1 - 0.5ml, though larger volumes may be processed by first incubating the antibody sample (combined with the 10X AbPure Binding Buffer) with the Protein A resin in a larger vessel (e.g. 2ml microcentrifuge tube) prior to transferring to the spin cartridge in several aliquots, spinning down excess liquid each time.

Packaging, Storage & Formulations

Storage
Store at 4°C.

Kit Components

Components
  1. 4 or 12 additional collecting tubes
  2. 2 or 3 vials of Protein A resin
  3. 1 or 3 spin cartridge/collecting tube assemblies
  4. 1 vial of 10X AbPure Binding Buffer
  5. 1 vial of AbPure Elution Buffer
  6. 1 vial of AbPure Wash Buffer
  7. 1 vial of AbPure Neutralization Buffer

Notes

This prodcut is manufactured by Expedeon Inc and distributed by Novus Biologicals.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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Array 260-0010

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