ABpure™ Antibody Purification Kit Summary
Commercially available antibodies often contain substances (e.g. BSA and other proteins, glycine, tris, and azide) that interfere in conjugation reactions. The AbPure Antibody Purification System quickly removes these contaminants. It can also be used to purify antibodies from crude samples such as ascites fluid or immune serum. The antibody to be purified or cleaned up ideally is in a volume of 0.1ml to 0.5ml. 20 to 500ug of antibody can be purified in each run.
|Species ||Isotype ||Binding Strength |
|Rabbit ||IgG ||High |
|Human ||IgG ||High |
|Pig ||IgG ||High |
|Mouse ||IgG1 ||Low/Medium |
|Mouse ||IgG2a ||High |
|Mouse ||IgG2b ||High |
|Mouse ||IgG3 ||Low/Medium |
|Goat ||IgG ||Low |
|Sheep ||IgG ||Low |
|Rat ||IgG ||Low |
Antibody Purification Kit
The volume of antibody to be purified or cleaned up should be 0.1 - 0.5ml, though larger volumes may be processed by first incubating the antibody sample (combined with the 10X AbPure Binding Buffer) with the Protein A resin in a larger vessel (e.g. 2ml microcentrifuge tube) prior to transferring to the spin cartridge in several aliquots, spinning down excess liquid each time.
Packaging, Storage & Formulations
- 4 or 12 additional collecting tubes
- 2 or 3 vials of Protein A resin
- 1 or 3 spin cartridge/collecting tube assemblies
- 1 vial of 10X AbPure Binding Buffer
- 1 vial of AbPure Elution Buffer
- 1 vial of AbPure Wash Buffer
- 1 vial of AbPure Neutralization Buffer
This prodcut is manufactured by Expedeon Inc and distributed by Novus Biologicals.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed
for 6 months from date of receipt.
Publications for ABpure (TM) Kit (260-0010) (0)
There are no publications for ABpure (TM) Kit (260-0010).
By submitting your publication information earn gift cards and discounts for future purchases.
Reviews for ABpure (TM) Kit (260-0010) (0)
There are no reviews for ABpure (TM) Kit (260-0010).
By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
- Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
- Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
FAQs for ABpure (TM) Kit (260-0010) (0)
Other Available Formats
Additional ABpure (TM) Products
Blogs on ABpure (TM)