A-549 Nuclear Cell Lysate

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Concentration
2 mg/ml

Order Details

A-549 Nuclear Cell Lysate Summary

Description
Nuclear extract cell lysate (denatured)
Localization
Lung
Specificity
A-549 (human lung carcinoma) nuclear extract lysate (denatured)
Preparation
Method
Nuclear extract was prepared by using a modified protocol of Dignam et al. Cells were Harvested and homogenized in Buffer A, and then centrifugated at 25,000 g for 20 minutes to remove cytoplasm and pellet the nuclei. The pellet was re-suspended in Buffer C, and then the suspensions were centrifuged to collect nuclear extract. The supernatant was dialyzed against Buffer D. The dialysate was then centrifuged, divided into aliquots, and stored at -80C. The protein concentration was determined by the method of Bradford. The lysate was adjusted to 2.5 mg/ml, and then mixed with 5X Sample Buffer to become final 2 mg/ml in 1X Sample Buffer. The lysate was heated at 95C for 5 min, and cooled rapidly.

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
The lysate is ready to load on SDS-PAGE for Western blotting.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
In ready-to-use 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue).
Concentration
2 mg/ml

Lysate Details for Array

Type
Cell
Tissue
Lung
Protein State
Denatured
Subcellular Fraction
Nuclear

Notes

Quality control test: 12.5% SDS-PAGE Stained with Coomassie Blue.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Video Protocols

WB Video Protocol

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Additional A-549 Products

Array L025V3

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