A-431 Nuclear Cell Lysate Summary
Nuclear extract cell lysate (denatured)
Nuclear extract was prepared by using a modified protocol of Dignam et al. Cells were Harvested and homogenized in Buffer A and then centrifugated at 25000 g for 20 minutes to remove cytoplasm and pellet the nuclei. The pellet was re-suspended in Buffer C and then the suspensions were centrifuged to collect nuclear extract. The supernatant was dialyzed against Buffer D. The dialysate was then centrifuged divided into aliquots and stored at -80C. The protein concentration was determined by the method of Bradford. The lysate was adjusted to 2.5 mg/ml and then mixed with 5X Sample Buffer to become final 2 mg/ml in Sample Buffer. The lysate was heated at 95C for 5 min and cooled rapidly.
Packaging, Storage & Formulations
Store at -80C. Avoid freeze-thaw cycles.
50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue
Lysate Details for Array
Quality control test: 12.5% SDS-PAGE Stained with Coomassie Blue.
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed
for 6 months from date of receipt.
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