8-OHdG ELISA Kit (Colorimetric)

ELISA: 8-OHdG ELISA Kit (Colorimetric) [NBP2-59714] - Diagram of the Triplicate Sample Plate Format

ELISA: 8-OHdG ELISA Kit (Colorimetric) [NBP2-59714] - Typical Standard Curve for the 8-OHdG ELISA kit (Enzyme-Linked Immunosorbent Assay) - NBP2-59714. Assay Type: Competitive ELISA. Detection Method: Colorimetric Assay. Assay Range: 0.94 - 60 ng/ml.

ELISA: 8-OHdG ELISA Kit (Colorimetric) [NBP2-59714] - Diagram of the 8-OHdG Competitive ELISA

Product Image: 8-OHdG ELISA Kit (Colorimetric) [NBP2-59714] - Chemical Equation of the Oxidation of Guanosine

Product Discontinued

8-OHdG ELISA Kit (Colorimetric) Summary

• Standard Curve Range: 0.94 - 60 ng/mL
• Sensitivity: 0.59 ng/mL
• Assay Type: Competitive ELISA
• Intra-Assay: Three samples of known concentration were assayed thirty times on one plate; the intra-assay coefficient of variation of the DNA Damage ELISA has been determined to be <5%. Inter-Assay Precision: Three samples of known concentration were assayed thirty ti
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA
  • Product Image
• Application Notes: ELISA Kit for 8-OHdG detection in samples.
  No of Samples: 39 samples in duplicate
  Incubation Time: 1 hour

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Alternate Names for 8-OHdG ELISA Kit (Colorimetric)

• 8 OHG
• 8-hydroxy-2
• 8-hydroxydeoxyguanosine

Background

8-hydroxy-2-deoxy Guanosine (8-OH-dG) is produced by the oxidative damage of DNA by reactive oxygen and nitrogen species and serves as an established marker of oxidative stress (1-4). Hydroxylation of guanosine occurs in response to both normal metabolic processes and a variety of environmental factors (i.e., anything that increases reactive oxygen and nitrogen species). Increased levels of 8-OH-dG are associated with the aging process as well as with a number of pathological conditions including cancer, diabetes, and hypertension(5-9). In complex samples such as plasma, cell lysates, and tissues, 8-OH-dG can exist as either the free nucleoside or incorporated in DNA. Once the blood enters the kidney, free 8-OH-dG is readily filtered into the urine, while larger DNA fragments remain in the bloodstream. Because of the complexity of plasma samples, urine is a more suitable matrix for the measurement of free 8-OH-dG than plasma. Urinary levels of 8-OH-dG range between 2.7-13 ng/mg creatine, while plasma levels of free 8-OH-dG have been reported to be between 4-21 pg/ml as determined by LC-MS (10-11).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for 8-OHdG ELISA Kit (NBP2-59714). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.