8-OHdG ELISA Kit (Colorimetric)

ELISA: 8-OHdG ELISA Kit (Colorimetric) [NBP2-59714] - Diagram of the Triplicate Sample Plate Format

ELISA: 8-OHdG ELISA Kit (Colorimetric) [NBP2-59714] - Typical Standard Curve for the 8-OHdG ELISA kit (Enzyme-Linked Immunosorbent Assay) - NBP2-59714. Assay Type: Competitive ELISA. Detection Method: Colorimetric Assay. Assay Range: 0.94 - 60 ng/ml.

ELISA: 8-OHdG ELISA Kit (Colorimetric) [NBP2-59714] - Diagram of the 8-OHdG Competitive ELISA

Product Image: 8-OHdG ELISA Kit (Colorimetric) [NBP2-59714] - Chemical Equation of the Oxidation of Guanosine

Product Discontinued

8-OHdG ELISA Kit (Colorimetric) Summary

• Standard Curve Range: 0.94 - 60 ng/mL
• Sensitivity: 0.59 ng/mL
• Assay Type: Competitive ELISA
• Intra-Assay: Three samples of known concentration were assayed thirty times on one plate; the intra-assay coefficient of variation of the DNA Damage ELISA has been determined to be <5%. Inter-Assay Precision: Three samples of known concentration were assayed thirty ti
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA
  • Product Image
• Application Notes: ELISA Kit for 8-OHdG detection in samples.
  No of Samples: 39 samples in duplicate
  Incubation Time: 1 hour

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Alternate Names for 8-OHdG ELISA Kit (Colorimetric)

• 8 OHG
• 8-hydroxy-2
• 8-hydroxydeoxyguanosine

Background

8-hydroxy-2-deoxy Guanosine (8-OH-dG) is produced by the oxidative damage of DNA by reactive oxygen and nitrogen species and serves as an established marker of oxidative stress (1-4). Hydroxylation of guanosine occurs in response to both normal metabolic processes and a variety of environmental factors (i.e., anything that increases reactive oxygen and nitrogen species). Increased levels of 8-OH-dG are associated with the aging process as well as with a number of pathological conditions including cancer, diabetes, and hypertension(5-9). In complex samples such as plasma, cell lysates, and tissues, 8-OH-dG can exist as either the free nucleoside or incorporated in DNA. Once the blood enters the kidney, free 8-OH-dG is readily filtered into the urine, while larger DNA fragments remain in the bloodstream. Because of the complexity of plasma samples, urine is a more suitable matrix for the measurement of free 8-OH-dG than plasma. Urinary levels of 8-OH-dG range between 2.7-13 ng/mg creatine, while plasma levels of free 8-OH-dG have been reported to be between 4-21 pg/ml as determined by LC-MS (10-11).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.