Rat 8-hydroxy-(2)-deoxyguanosine ELISA Kit (Colorimetric)

ELISA: 8-hydroxy-(2)-deoxyguanosine ELISA Kit [HRP] [KA0444] - Standard curve

Product Discontinued

Rat 8-hydroxy-(2)-deoxyguanosine ELISA Kit (Colorimetric) Summary

• Description: Quality control test: Standard curve. 8-hydroxy-2'-deoxyguanosine ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of 8-hydroxy-2'-deoxyguanosine.
• Standard Curve Range: 0.5 to 200 ng/mL
• Sensitivity: 0.5 ng/mL
• Assay Type: Sandwich ELISA
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA
  • Functional
  • Quantification
• Application Notes: Useful for quantification.

Reactivity Notes

Rat reactivity reported in multiple pieces of scientific literature

Packaging, Storage & Formulations

• Storage: Store at 4C.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for 8-hydroxy-(2)-deoxyguanosine ELISA Kit (KA0444)(11)

Publications using KA0444

• Shedid SM, Abdel-Magied N, Saada HN. et al. Role of betaine in liver injury induced by the exposure to ionizing radiation. Environ Toxicol 2017-10-11 [PMID: 30311401]
• Awad A, Khalil SR, Farag MR et al. Differential susceptibility of kidneys and livers to proliferative processes and transcriptional level of the genes encoding desmin, vimentin, connexin 43, and nestin in rats exposed to furan. Ecotoxicol Environ Saf 2018-07-06 [PMID: 29990736]
• Abo El-Khair SM, Gaballah MA, Abdel-Gawad MM et al. Spermatozoal Fractalkine Signaling Pathway Is Upregulated in Subclinical Varicocele Patients with Normal Seminogram and Low-Level Leucospermia. Adv Urol 2017-12-21 [PMID: 29527225]
• Abu Zeid EH, Hussein MMA, Ali H. Ascorbic acid protects male rat brain from oral potassium dichromate-induced oxdative DNA damage and apoptotic changes: the expression patterns of caspase-3, P 53, Bax, and Bcl-2 genes. Environ Sci Pollut Res Int 2018-05-01 [PMID: 29484617] (ELISA, Rat)
• Attia H, Al-Rasheed N, Mohamad R et al. The antifibrotic and fibrolytic properties of date fruit extract via modulation of genotoxicity, tissue-inhibitor of metalloproteinases and nuclear factor- kappa B pathway in a rat model of hepatotoxicity. BMC Complement Altern Med 2016-10-24 [PMID: 27776513]
• Schlegel A, Kron P, Graf R et al. Hypothermic Oxygenated Perfusion (HOPE) downregulates the immune response in a rat model of liver transplantation. Ann Surg. 2014-11-01 [PMID: 25243553] (Rat)
• Schlegel A, Kron P, Graf R et al. Warm vs. cold perfusion techniques to rescue rodent liver grafts. J Hepatol. 2014-12-01 [PMID: 25086285] (Rat)
• Schlegel A, Graf R, Clavien PA, Dutkowski P. Hypothermic oxygenated perfusion (HOPE) protects from biliary injury in a rodent model of DCD liver transplantation. J Hepatol. 2013-11-01 [PMID: 23820408] (Rat)
• Kantha SS, Wada S, Tanaka H et al. Carnosine sustains the retention of cell morphology in continuous fibroblast culture subjected to nutritional insult. Biochem Biophys Res Commun. 1996-06-14 [PMID: 8670272]
• Kasai H, Crain PF, Kuchino Y et al. Formation of 8-hydroxyguanine moiety in cellular DNA by agents producing oxygen radicals and evidence for its repair. Carcinogenesis. 1986-11-01 [PMID: 3769133]
• Fraga CG, Shigenaga MK, Park JW et al. Oxidative damage to DNA during aging: 8-hydroxy-2'-deoxyguanosine in rat organ DNA and urine. Proc Natl Acad Sci U S A. 1990-06-01 [PMID: 2352934]

FAQs for 8-hydroxy-(2)-deoxyguanosine ELISA Kit (KA0444). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.