7-Aminoactinomycin D Solution

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Flow Cytometry: 7-Aminoactinomycin D Solution [NBP2-29446] - Mouse lymphocytes were unstained (left) or stained (right) with 7-AAD. Without 7-AAD, dead cells cannot be visualized. With 7-AAD, dead cells can be ...read more
7-Aminoactinomycin D Solution [NBP2-29446] - 7-Aminoactinomycin D [NBP2-29446]

Product Details

Summary
Applications Flow

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7-Aminoactinomycin D Solution Summary

Description
Appearance (Form) Powder
Appearance (Color) Red to Dark Purple
Solubility (Solvent) Chloroform
Solubility (Conc) 0.95 - 1.05 mg/ml
Solubility (Turbidity) Clear
Solubility (Color) Red
Purity (HPLC) > 95.50%
Specificity
7-Aminoactinomycin D Solution
Molecular Weight 1270.43

Applications/Dilutions

Application Notes
Fluorescent DNA stain
7- Amino-Actinomycin D (7-AAD) can be used as a viability probe for methods of nonviable cell exclusion based on light scatter and uptake of the reagent as detected in FL3. 7-AAD is excluded by viable cells but can penetrate cell membranes of dying or dead cells, in which it intercalates into double stranded nucleic acids. It can be used in place of Propidium Iodide (PI) for omission of nonviable cells in flow cytometric assay. The advantage of 7-AAD over PI is that detection is in the far red range of the spectrum (650 nm long-pass filter) and is read in the FL3, compared to PI detection in FL2. This allows this solution to be used in combination with PE (phycoerythrin), FITC (fluorescein isothiocyanate), and APC (allophycocyanin) conjugated antibodies, as well as any other conjugated antibody that does not emit into FL3.
Publications
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NBP2-29446 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS and 1.0% FBS
Preservative
0.09% Sodium Azide

Alternate Names for 7-Aminoactinomycin D Solution

  • 7-AAD
  • C62H87N13O16

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

Publications for 7-Aminoactinomycin D Solution (NBP2-29446)(2)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: Flow-IC.


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