6-Keto-Prostaglandin F1A ELISA Kit (Colorimetric)

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ELISA: 6-Keto-Prostaglandin F1A ELISA Kit (Colorimetric) [NBP2-61286] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Product Details

Summary
Applications ELISA
Suitable Sample Type
Serum, saliva, and urine from any species
Standard Curve Range
3.2 - 50,000 pg/ml
Sensitivity
1.40 pg/ml

Order Details

6-Keto-Prostaglandin F1A ELISA Kit (Colorimetric) Summary

Standard Curve Range
3.2 - 50,000 pg/ml
Sensitivity
1.40 pg/ml
Assay Type
Competitive ELISA
Inter-Assay
Mean 6.3%
Intra-Assay
Mean 6.9%
Spike Recovery
Tissue Culture Media - 106.7%
Human Saliva - 110.7%
Human Urine - 108.7%
Human Serum - 108.4%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Store at 4°C.

Kit Components

Components
  1. Antibody
  2. Assay buffer
  3. Conjugate
  4. DxS IgG Microtiter plate
  5. Standard, pNpp Substrate
  6. Stop solution
  7. Wash buffer concentrate

Background

6-keto-PGF1a is a stable hydrolysis product of prostacyclin (PGI2). Due to the short half-life of PGI2 in buffer (2-3 minutes) the production of prostacyclin is typically monitored by measurement of the 6-keto-PGF1a metabolite and its derivative in urine, 2,3-dinor-6-keto-PGF1a. PGI2 has autocoid functions that include platelet aggregation, inhibition of vasoconstrictor responses to pressor hormones, platelet anti- and disaggregatory properties, and reproductive functions. The effects are induced by receptor-mediated elevation of cAMP. Prostacyclin has clinical applications in atherosclerosis and protection from myocardial ischemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for 6-Keto-Prostaglandin F1A ELISA Kit (NBP2-61286). (Showing 1 - 1 of 1 FAQ).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

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