6-Keto-Prostaglandin F1A ELISA Kit (Colorimetric)

ELISA: 6-Keto-Prostaglandin F1A ELISA Kit (Colorimetric) [NBP2-61286] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

• Applications: ELISA
• Suitable Sample Type: Serum, saliva, and urine from any species
• Standard Curve Range: 3.2 - 50,000 pg/ml
• Sensitivity: 1.40 pg/ml

6-Keto-Prostaglandin F1A ELISA Kit (Colorimetric) Summary

• Standard Curve Range: 3.2 - 50,000 pg/ml
• Sensitivity: 1.40 pg/ml
• Assay Type: Competitive ELISA
• Inter-Assay: Mean 6.3%
• Intra-Assay: Mean 6.9%
• Spike Recovery: Tissue Culture Media - 106.7%
  Human Saliva - 110.7%
  Human Urine - 108.7%
  Human Serum - 108.4%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Store at 4°C.

Background

6-keto-PGF1a is a stable hydrolysis product of prostacyclin (PGI2). Due to the short half-life of PGI2 in buffer (2-3 minutes) the production of prostacyclin is typically monitored by measurement of the 6-keto-PGF1a metabolite and its derivative in urine, 2,3-dinor-6-keto-PGF1a. PGI2 has autocoid functions that include platelet aggregation, inhibition of vasoconstrictor responses to pressor hormones, platelet anti- and disaggregatory properties, and reproductive functions. The effects are induced by receptor-mediated elevation of cAMP. Prostacyclin has clinical applications in atherosclerosis and protection from myocardial ischemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for 6-Keto-Prostaglandin F1A ELISA Kit (NBP2-61286). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.