25-hydroxy Vitamin D3 ELISA Kit (Colorimetric)

Universal 25-hydroxy Vitamin D3 ELISA Kit (Colorimetric) [NBP2-66361] - Standard Curve Reference

ELISA: 25-hydroxy Vitamin D3 ELISA Kit (Colorimetric) [NBP2-66361] - Samples were spiked with high concentrations of 25-hydroxy Vitamin D3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: 25-hydroxy Vitamin D3 ELISA Kit (Colorimetric) [NBP2-66361] - Standard Curve Reference

• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 3.13 - 200 ng/mL
• Sensitivity: 1.88 ng/mL

25-hydroxy Vitamin D3 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes 25-hydroxy Vitamin D3 in samples. No significant cross-reactivity or interference between 25-hydroxy Vitamin D3 and analogues was observed.
• Standard Curve Range: 3.13 - 200 ng/mL
• Sensitivity: 1.88 ng/mL
• Assay Type: Competitive-ELISA
• Inter-Assay: CV% < 4.96%
• Intra-Assay: CV% < 5.02%
• Spike Recovery: 87-112%
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for 25-hydroxy Vitamin D3 ELISA Kit (Colorimetric)

• 25-Hydroxycholecalciferol
• 25-Hydroxyvitamin D
• 25-hydroxyvitamin D3
• 25-OH D3
• 25-OH Vitamin D3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for 25-hydroxy Vitamin D3 ELISA Kit (NBP2-66361)(1)

Publication using NBP2-66361

• Alabdullatif W, Almnaizel A, Alhijji A et al. Correlation of Plasma 25(OH)D3 and Vitamin D Binding Protein Levels with COVID-19 Severity and Outcome in Hospitalized Patients Nutrients 2023-04-10 [PMID: 37111039] (ELISA)