Human 20S Proteasome ELISA Kit (Colorimetric)

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ELISA: Human 20S Proteasome ELISA Kit (Colorimetric) [NBP2-62173] - The ELISA plate is coated with the capture antibody and the proteasome sample is added and allowed to complex with the bound antibody. The detection ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Biological samples
Standard Curve Range
25 - 1600 ng/mL
Sensitivity
25 ng/mL

Order Details

Human 20S Proteasome ELISA Kit (Colorimetric) Summary

Description
Kit is not suitable for mouse or rat samples.
Standard Curve Range
25 - 1600 ng/mL
Sensitivity
25 ng/mL
Assay Type
Sandwich ELISA
Inter-Assay
N/A
Intra-Assay
N/A
Spike Recovery
N/A
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.

Kit Components

Components
  1. Binding Buffer
  2. Blocking Buffer
  3. Capture Antibody
  4. Conjugated Antibody
  5. Detection Antibody
  6. ELISA Buffer
  7. Lysis Buffer
  8. Plate
  9. Proteasome Stock Solution
  10. Stop Solution
  11. TMB Substrate Solution
  12. Wash Buffer

Alternate Names for Human 20S Proteasome ELISA Kit (Colorimetric)

  • 20S Proteasome

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for 20S Proteasome ELISA Kit (NBP2-62173). (Showing 1 - 1 of 1 FAQ).

  1. What is the difference between the Quantikine and Duoset ELISA kits?
    • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

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