Human 17-Beta-Estradiol ELISA Kit (Colorimetric)


ELISA: 17-Beta-Estradiol ELISA Kit [Alkaline Phosphatase] [KA0297] - Standard curve

Product Details

Reactivity HuSpecies Glossary
Applications ELISA, QFN
Suitable Sample Type
Saliva and Tissue culture media
Standard Curve Range
29.3 to 30000 pg/mL
28.5 pg/mL

Order Details

Human 17-Beta-Estradiol ELISA Kit (Colorimetric) Summary

17 beta-Estradiol ELISA Kit is a competitive immunoassay for the quantitative determination of 17 beta-Estradiol in biological and environmental samples.
Standard Curve Range
29.3 to 30000 pg/mL
28.5 pg/mL
Assay Type
Competitive ELISA
CV% < 6.20%
CV% < 8.60%
Spike Recovery
Cell Culture Supernatant: 92.20%, Saliva: 104.80%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)


  • Quantification
Application Notes
Useful for quantification.
Read Publication using KA0297.

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 23465317)

Packaging, Storage & Formulations

Store at 4C.

Kit Components

  1. 17 beta -Estradiol ELISA Antibody: A yellow solution of a rabbit polyclonal antibody to 17 beta -Estradiol.
  2. 17 beta -Estradiol ELISA Conjugate: A blue solution of alkaline phosphatase conjugated with17 beta -Estradiol.
  3. 17 beta -Estradiol Standard: A solution of 300,000 pg/mL 17 beta -Estradiol.
  4. Assay Buffer 3: Tris buffered saline containing proteins and detergents and sodium azide as apreservative
  5. Goat anti-Rabbit IgG Microtiter Plate: A plate using break-apart strips coated with goat antibodyspecific to rabbit IgG.
  6. Plate Sealer
  7. pNpp Substrate: A solution of p-nitrophenyl phosphate in buffer. Ready to use.
  8. Steroid Displacement Reagent: A special formulated displacer to inhibit steroid binding toproteins.
  9. Stop Solution: A solution of trisodium phosphate in water. Keep tightly capped. Caution: Caustic
  10. Wash Buffer Concentrate: Tris buffered saline containing detergents.


Quality control test: Standard curve.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Human 17-Beta-Estradiol ELISA Kit (Colorimetric)

  • 17 beta Oestradiol


This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for 17-Beta-Estradiol ELISA Kit (KA0297)(1)

We have publications tested in 1 confirmed species: Human.

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